Application Data
(Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor647)
Mouse CD63 Monoclonal Antibody | anti-CD63 antibody
MOUSE ANTI RAT CD63
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
CD63, Antibody; MOUSE ANTI RAT CD63; anti-CD63 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AD1
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-CD63 antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
Immunogen
RBL-2H3 rat basophilic leukemia cell line.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag8-653 myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor647)
Application Data
(Rat peritoneal macrophages stained with Mouse anti Rat CD63:Alexa Fluor647)
Application Data
(Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor488)
Application Data
(Rat peripheral blood platelets stained with Mouse anti Rat CD63 Alexa Fluor488)
Application Data
(Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC)
Application Data
(Rat peripheral blood platelets stained with Mouse anti Rat CD63:FITC)
Related Product Information for anti-CD63 antibody
Mouse anti Rat CD63 antibody, clone AD1 specifically recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, which is translocated to the cell surface upon activation of platelets, and therefore a potential indicator of platelet activation. Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1. Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release (Kitani et al. 1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD63 antigen
NCBI Official Synonym Full Names
Cd63 molecule
NCBI Official Symbol
Cd63
NCBI Protein Information
CD63 antigen; mast cell antigen AD1
UniProt Protein Name
CD63 antigen
UniProt Gene Name
Cd63
UniProt Entry Name
CD63_RAT
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Product Notes
The CD63 cd63 (Catalog #AAA49778) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD63 cd63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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