IF (Immunofluorescence)
(Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3 Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of NK-1-C3 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human, Mouse CD63 Monoclonal Antibody | anti-CD63 antibody
Anti-CD63 [NK-1-C3]
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay, Western Blot
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD63, Antibody; Anti-CD63 [NK-1-C3]; Granulophysin; LAMP-3; Tetraspanin-30; Tspan-30; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; CD63 antigen; NK1/C3; NKI/C3; NK-1.C3; NK1C3; anti-CD63 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
NK-1-C3
Specificity
This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD63 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot)
PMID
3882581
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3 Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of NK-1-C3 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3 Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of NK-1-C3 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CD63 antibody NK-1-C3 K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of NK-1-C3 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CD63 antibody NK-1-C3 K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of NK-1-C3 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-CD63 antibody
Recombinant monoclonal antibody to CD63. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NK-1-C3.
This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
Product Categories/Family for anti-CD63 antibody
References
Vennegoor et al. Biochemical characterization and cellular localization of a formalin-resistant melanoma-associated antigen reacting with monoclonal antibody NKI/C-3. Int J Cancer. 1985 Mar 15;35(3):287-95.
Note on publication: This article describes the generation and characterization of this anntibody (NKI/C-3).
Note on publication: This article describes the generation and characterization of this anntibody (NKI/C-3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,430 Da
NCBI Official Full Name
melanoma-associated antigen
NCBI Official Synonym Full Names
CD63 molecule
NCBI Official Symbol
CD63
NCBI Official Synonym Symbols
MLA1; ME491; LAMP-3; OMA81H; TSPAN30
NCBI Protein Information
CD63 antigen
UniProt Protein Name
CD63 antigen
UniProt Gene Name
CD63
UniProt Synonym Gene Names
MLA1; TSPAN30; LAMP-3; Tspan-30
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Product Notes
The CD63 cd63 (Catalog #AAA72163) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD63 [NK-1-C3] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD63 cd63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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