FCM/FACS (Flow Cytometry)
CD66b (CEACAM8) Monoclonal Antibody | anti-CD66b antibody
Anti-Human CD66b (CEACAM8)
Gene Names
CEACAM8; CD67; CGM6; CD66b; NCA-95
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
CD66b (CEACAM8), Antibody; Anti-Human CD66b (CEACAM8); anti-CD66b (ANC4A4)FITC Conjugate; Monoclonal anti-human CD66b (CEACAM8)/FITC*; anti-CD66b antibody
Clonality
Monoclonal
Isotype
IgG1K
Clone Number
ANC4A4
Form/Format
FITC; 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Sequence Length
349
Applicable Applications for anti-CD66b antibody
ELISA, FCM/FACS (Flow Cytometry)
Immunogen
Recombinant soluble human CD66b
Performance
Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD66b(ANC4A4) /FITC at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 54% sub population of the cells stained positive with a mean shift of 1.05 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD66b(ANC4A4) antibody.
Production
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at approximately 0.5 mg/ml with a Fluorescein/IgG molar ratio of 7.4
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-CD66b antibody
Human CD66b (CEACAM8, CGMb, NCA-95, CD67) is a 95kD member of the Carcinoembryonic Antigen (CEA) group. It is expressed on granulocytes and functions as an adhesion molecule. Similar to the CD66 a, c, and d isoforms, engagement of CD66b on neutrophils causes increased adhesion to endothelial cells (2). Crosslinking CD66b on neutrophils with antibodies triggers IL-8 release (3).
Antibody from clone ANC3C5 binds to CD66b on the cell surface of human PMN in Flow cytometry and works as an EIA capture or detection reagent for recombinant CD66b when paired with clone ANC4A4(Cat MBS666902).
Antibody from clone ANC3C5 binds to CD66b on the cell surface of human PMN in Flow cytometry and works as an EIA capture or detection reagent for recombinant CD66b when paired with clone ANC4A4(Cat MBS666902).
Product Categories/Family for anti-CD66b antibody
References
1) W.N. Khan, S. Hammarstrom, et al. (1992) Genomics 14(2):384-90. 2) K.M. Skubitz, A.P. Skubitz, et al. (1996) J Leuk Biol 60(1): 106-117. 3) A.K. Schroder, L Rink, et al. (2006) Human Immunol 67(9): 676-682.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Gene Name
CEACAM8
UniProt Synonym Gene Names
CGM6
UniProt Entry Name
CEAM8_HUMAN
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Product Notes
The CD66b ceacam8 (Catalog #AAA78197) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD66b (CEACAM8) can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD66b ceacam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD66b (CEACAM8), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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