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WB (Western Blot)
(Detection of human CEACAM8/CD66b by western blot. Samples: Whole cell lysate (10 ug) from Raji, K-562, HL-60, THP-1, KG-1, MUTZ-3, SR, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
CEACAM8/CD66b recombinant antibody
Rabbit anti-CEACAM8/CD66b Recombinant Monoclonal Antibody [BLR111H]
Gene Names
CEACAM8; CD67; CGM6; CD66b; NCA-95
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CEACAM8/CD66b, Recombinant Antibody; Rabbit anti-CEACAM8/CD66b Recombinant Monoclonal Antibody [BLR111H]; carcinoembryonic antigen-related cell adhesion molecule 8; NCA-95; CGM6; CD67 antigen; CD67; CD antigen CD66b; non-specific cross-reacting antigen NCA-95; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen CGM6; CD66b; Carcinoembryonic antigen-related cell adhesion molecule 8; carcinoembryonic antigen related cell adhesion molecule 8; CEACAM8/CD66b recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR111H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for CEACAM8/CD66b recombinant antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 23-320 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CEACAM8/CD66b by western blot. Samples: Whole cell lysate (10 ug) from Raji, K-562, HL-60, THP-1, KG-1, MUTZ-3, SR, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human CEACAM8/CD66b by western blot. Samples: Whole cell lysate (10 ug) from Raji, K-562, HL-60, THP-1, KG-1, MUTZ-3, SR, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CEACAM8/CD66b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HL-60 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 lot 1) used at 20 ul/mg lysate. For blotting immunoprecipitated CEACAM8/CD66b, AAA213582 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CEACAM8/CD66b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HL-60 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 lot 1) used at 20 ul/mg lysate. For blotting immunoprecipitated CEACAM8/CD66b, AAA213582 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of human CEACAM8/CD66b in FFPE lung carcinoma pleural effusion by immunohistochemistry. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CEACAM8/CD66b in FFPE lung carcinoma pleural effusion by immunohistochemistry. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CEACAM8/CD66b in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CEACAM8/CD66b in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody (AAA213582 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for CEACAM8/CD66b recombinant antibody
CEACAM8/CD66b member of the CEACAM subfamily of the CEA family of proteins. It is a cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. It is expressed by neutrophils and eosinophils, and mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P31997].
Product Categories/Family for CEACAM8/CD66b recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,154 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Gene Name
CEACAM8
UniProt Synonym Gene Names
CGM6
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Product Notes
The CEACAM8/CD66b ceacam8 (Catalog #AAA213582) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CEACAM8/CD66b Recombinant Monoclonal Antibody [BLR111H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM8/CD66b can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CEACAM8/CD66b ceacam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM8/CD66b, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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