Rat CD68 Monoclonal Antibody | anti-CD68 antibody
RAT ANTI MOUSE CD68:Endotoxin Low
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. We recommends the use of Leucoperm for this purpose.
Immunohistology - Paraffin: Application Note: This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
Western Blotting: Application Note: Non-reducing conditions recommended.
Buffer Solution
Shelf Life: 18 months from date of despatch.
Application Data
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)
Application Data
(Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)
Application Data
(Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa FluorR 647 . following permeablisation with Leucoperm)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: RPE . following permeablisation with Leucoperm)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa FluorR 488 . following permeablisation with Leucoperm)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 , following permeablisation with Leucoperm)
Application Data
(Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed))
Application Data
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC . following permeablisation with Leucoperm)