FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD68antibodyFA-11(AAA72504) Mousesplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)ortherabbitIgG-chimericversionofFA-11(AAA72504,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
CD68 recombinant antibody
Anti-CD68 [FA-11], Rabbit IgG, kappa
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD68, Recombinant Antibody; Anti-CD68 [FA-11], Rabbit IgG, kappa; CD68; Anti-CD68 [FA-11]; Macrosialin; FA/11; FA11 antigen; CD68 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
FA-11
Specificity
This antibody is specific for murine CD68, known also as macrosialin (MS). CD68 is a 110 kDa, heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the lysosomal/endosomal-associated membrane glycoproteins (LAMP). CD68 is predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several acute myeloid leukemia studies.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD68 recombinant antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD68antibodyFA-11(AAA72504) Mousesplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)ortherabbitIgG-chimericversionofFA-11(AAA72504,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-CD68antibodyFA-11(AAA72504) Mousesplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,blackline)ortherabbitIgG-chimericversionofFA-11(AAA72504,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
WB (Western Blot)
(Western Blotusinganti-CD68antibodyFA-11(AAA72504) RAW-264.7cells(A),mousespleen(B)lysates(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofFA-11(AAA72504)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-CD68antibodyFA-11(AAA72504) RAW-264.7cells(A),mousespleen(B)lysates(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofFA-11(AAA72504)at1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
Related Product Information for CD68 recombinant antibody
This antibody was raised by immunising rats (AO x Lou strain) with murine macrosialin (MS).
References
Smith et al. Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes. J Cell Sci. 1987 Feb;87 (Pt 1):113-9. N/A3312248
Note on publication:
Describes the original generation of this antibody and its subsequent applications in binding assays, immunoprecipitation and immunofluorescence to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes.
Note on publication:
Describes the original generation of this antibody and its subsequent applications in binding assays, immunoprecipitation and immunofluorescence to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,844 Da
NCBI Official Full Name
Macrosialin
NCBI Official Synonym Full Names
CD68 antigen
NCBI Official Symbol
Cd68
NCBI Official Synonym Symbols
Lamp4; gp110; Scard1
NCBI Protein Information
macrosialin
UniProt Protein Name
Macrosialin
UniProt Gene Name
Cd68
UniProt Entry Name
CD68_MOUSE
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Product Notes
The CD68 cd68 (Catalog #AAA72504) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD68 [FA-11], Rabbit IgG, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD68 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD68 cd68 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD68, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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