Mouse anti-Human CD7 Monoclonal Antibody | anti-CD7 antibody

CD7

Cat. Num. AAA13566

• Gene Names: CD7; GP40; TP41; Tp40; LEU-9
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: CD7, Antibody; CD7; anti-CD7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: LP15
• Form/Format: CD7 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Applicable Applications for anti-CD7 antibody: Immunohistochemistry (IHC) - Paraffin, Frozen.
• Application Notes: Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations.; ; Specimen Preparation:; ; Paraffin sections:; The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.; Frozen sections and cell preparations:; The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Localization: Membranous
• Control: Tonsil, Lymph Node
• Immunogen: Full length of the human CD7 protein.
• Precautions: 1. This product contains <0.1% sodium azide (NaN₃) as a preservative. Ensure proper handling procedures are used with this reagent.; 2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 3. Dispose of unused solution with copious amount of water.; 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 7. For additional safety information please request Data Sheet for this product.; 8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document)
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. ; 2. Air dry for 2 hours at 58° C. ; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate. ; 5. Any of three heating methods may be used: ; a. TintoRetriever Pressure Cooker or Equivalent:; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. ; b. TintoRetriever PT Module or Water Bath Method:; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. ; c. Conventional Steamer Method:; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with IHC wash buffer or DI water.; 9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter (please inquire) or organic solvent based resin such as PermaMounter.
• Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.
• Preparation and Storage: Store at 2-8°C (Control Slides: Store at 20-25 degree C). Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

IHC Protocol

IHC (Immunohistochemistry)

(IHC of CD7 on a FFPE Tonsil Tissue)

Dilution Information

Related Product Information for anti-CD7 antibody

CD7 is a 40 kDa transmembrane, single-chain glycoprotein, which is a member of the immunoglobulin gene superfamily. It is expressed in the majority of immature and mature T-lymphocytes, and T-cell Leukemia. It is also found in natural killer cells, a small subpopulation of normal B-cells and in malignant B-cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

CD7 is a consistently-expressed T-cell antigen in Lymphoblastic Lymphomas and Leukemias; therefore, it is a useful marker in the identification of such neoplastic proliferations. CD7 is expressed in the majority of mature peripheral T-cells, the majority of post-thymic T-cells, NK cells, some myeloid cells, T-cell Acute Lymphoblastic Leukemia/Lymphoma, Acute Myelogenous Leukemia and Chronic Myelogenous Leukemia. Interestingly, CD7 is conspicuously absent in adult T-cell Leukemia/Lymphoma and is not expressed in Sezary cells.

NCBI and Uniprot Product Information

• NCBI GI #: 180164
• NCBI GeneID: 924
• UniProt Accession #: P09564; Q29VG2; Q29VG3; Q29VG4; Q29VG5; Q53F24
• NCBI Official Full Name: CD7 antigen protein
• NCBI Official Synonym Full Names: CD7 molecule
• NCBI Official Symbol: CD7
• NCBI Official Synonym Symbols: GP40; TP41; Tp40; LEU-9
• NCBI Protein Information: T-cell antigen CD7; p41 protein; CD7 antigen (p41); T-cell leukemia antigen; T-cell surface antigen Leu-9
• UniProt Protein Name: T-cell antigen CD7
• UniProt Gene Name: CD7
• UniProt Entry Name: CD7_HUMAN