Application Data
(Binding of anti-CD70/PE to human Raji cells)
Mouse CD70 Monoclonal Antibody | anti-CD70 antibody
anti-human CD70-R-PE
Gene Names
CD70; CD27L; CD27LG; TNFSF7
Applications
Immunohistochemistry, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
Synonyms
CD70, Antibody; anti-human CD70-R-PE; Monoclonal anti-human CD70/R-PE; anti-CD70 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BU69
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Sequence Length
193
Applicable Applications for anti-CD70 antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, FCM/FACS (Flow Cytometry)
Performance
Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD70/R-PE at a 1:50 dilution (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.33 log10 fluorescent units when compared to a Mouse IgG1/PE negative control ( ) at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD70 antibody ( )
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody aPE were removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with PE: Ab molar ratio of 1.21.
Immunogen
Human WM-1 (Waldenström’s macroglobulinemia) cell line
Preparation and Storage
Store at 2-5°C.
Do not freeze! Protect from light.
Product should retain activity for at least after shipping date when stored as recommended.
Do not freeze! Protect from light.
Product should retain activity for at least after shipping date when stored as recommended.
Application Data
(Binding of anti-CD70/PE to human Raji cells)
Application Data
(Binding of anti-CD70/PE to human Raji cells)
Application Data
Application Data
Related Product Information for anti-CD70 antibody
Information: Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. Antibody BU69 recognizes CD70 molecule of approximately 30 kd. Antibody BU69 inhibits T cell proliferation induced by dendritic cells.
References
R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 molecule
NCBI Official Symbol
CD70
NCBI Official Synonym Symbols
CD27L; CD27LG; TNFSF7
NCBI Protein Information
CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
UniProt Protein Name
CD70 antigen
UniProt Gene Name
CD70
UniProt Synonym Gene Names
CD27L; CD27LG; TNFSF7; CD27-L
UniProt Entry Name
CD70_HUMAN
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Product Notes
The CD70 cd70 (Catalog #AAA78149) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD70 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD70 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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