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FCM (Flow Cytometry)
(Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/106 cells of CD73 clone (ABM40E2). Green represents isotype control; red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
Mouse anti-Human CD73 Monoclonal Antibody | anti-CD73 antibody
CD73 Monoclonal Antibody
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein G Chromatography
Synonyms
CD73, Antibody; CD73 Monoclonal Antibody; anti-CD73 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b, Kappa
Clone Number
ABM40E2
Purity/Purification
Protein G Chromatography
Form/Format
Purified (Liquid)
Concentration
0.5 mg/ml, 200 ul (varies by lot)
Sequence Length
524
Applicable Applications for anti-CD73 antibody
Western Blot (WB), Immunohistochemistry (IHC), FACS
Application Notes
WB: 2 - 4ug/ml
IHC: 5ug/ml
FACS: 0.2 - 0.5ug/106 cells
IHC: 5ug/ml
FACS: 0.2 - 0.5ug/106 cells
Content
25 ug in 50 ul/100ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide.
Sodium azide is highly toxic.
Sodium azide is highly toxic.
Immunogen
A partial length recombinant CD73 protein (amino acids 145-346) was used as the immunogen for this antibody.
Preparation and Storage
Store antibody at 4°C, stable for up to 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
FCM (Flow Cytometry)
(Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/106 cells of CD73 clone (ABM40E2). Green represents isotype control; red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
FCM (Flow Cytometry)
(Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/106 cells of CD73 clone (ABM40E2). Green represents isotype control; red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in human spleen using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in human spleen using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in renal cell carcinoma using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in renal cell carcinoma using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in human liver using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in human liver using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in endomatrium carcinoma using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD73 in endomatrium carcinoma using CD73 antibody (Clone: ABM40E2) at 5 ug/ml.)
WB (Western Blot)
(Expression analysis of CD73. Anti- CD73 antibody (Clone: ABM40E2) was tested at 2 ug/ml on human liver lysate.)
WB (Western Blot)
(Expression analysis of CD73. Anti- CD73 antibody (Clone: ABM40E2) was tested at 2 ug/ml on human liver lysate.)
Related Product Information for anti-CD73 antibody
CD73, also known as ecto-5'-nucleotidase, is a glycosyl-phosphatidylinositol linked plasma membrane glycoprotein that is expressed on multiple cell types and in different tissues. It is a key enzyme in the regulation of purinergic signaling and inflammatory reactions. It hydrolyzes extracellular AMP into adenosine, which dampens immune cell activation, and reduces leukocyte trafficking CD73 potently suppresses antitumor T cell responses through its ability to generate adenosine. It has also emerged as an important regulator of tissue homeostasis and pathophysiologic responses related to immunity, inflammation, pain, ischemia, tissue fibrosis and cancer.
Product Categories/Family for anti-CD73 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
5'-nucleotidase isoform 2 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
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Product Notes
The CD73 nt5e (Catalog #AAA14868) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD73 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD73 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), FACS. WB: 2 - 4ug/ml IHC: 5ug/ml FACS: 0.2 - 0.5ug/106 cells. Researchers should empirically determine the suitability of the CD73 nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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