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WB (Western Blot)
(Detection of human CD73 by western blot. Samples: Whole cell lysate (50 ug) from NCI-H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
CD73 recombinant antibody
Rabbit anti-CD73 Recombinant Monoclonal Antibody [BLR054F]
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
CD73, Recombinant Antibody; Rabbit anti-CD73 Recombinant Monoclonal Antibody [BLR054F]; eN; NTE; NT5; CALJA; NT; Purine 5-Prime-Nucleotidase; eNT; E5NT; CD antigen CD73; 5'-nucleotidase; 5'-NT; CD73; ecto-5'-nucleotidase; 5'-nucleotidase ecto; CD73 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR054F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for CD73 recombinant antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Residues 27-552 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD73 by western blot. Samples: Whole cell lysate (50 ug) from NCI-H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
WB (Western Blot)
(Detection of human CD73 by western blot. Samples: Whole cell lysate (50 ug) from NCI-H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
IHC (Immunohistochemisry)
(Detection of human CD73 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemisry)
(Detection of human CD73 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohiostchemistry)
(Detection of human CD73 by immunohistochemistry. Sample: FFPE section of head and neck squamous cell carcinoma. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human CD73 by immunohistochemistry. Sample: FFPE section of head and neck squamous cell carcinoma. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD73 by immunocytochemistry. Sample: FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD73 by immunocytochemistry. Sample: FFPE section of IMR-90 cells. Antibody: Rabbit anti-CD73 recombinant monoclonal antibody (AAA213551 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for CD73 recombinant antibody
CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. CD73 is used as a determinant of lymphocyte differentiation. [provided by RefSeq, Mar 2011] [taken from NCBI Entrez Gene (Gene ID: 4907)].
Product Categories/Family for CD73 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,949 Da
NCBI Official Full Name
5'-nucleotidase isoform 1 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
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Product Notes
The CD73 nt5e (Catalog #AAA213551) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD73 Recombinant Monoclonal Antibody [BLR054F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD73 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD73 nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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