FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
Mouse anti-Human CD73 Monoclonal Antibody | anti-CD73 antibody
Anti-Hu CD73 APC-Cyanine7
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with tandem dye APC-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD73, Antibody; Anti-Hu CD73 APC-Cyanine7; 5'-nucleotidase ecto; ecto-5'nucleotidase; NT5E; E5NT; CALJA; NTE; anti-CD73 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AD2
Specificity
The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.
Purity/Purification
The purified antibody is conjugated with tandem dye APC-Cyanine7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
574
Applicable Applications for anti-CD73 antibody
FCM/FACS (Flow Cytometry)
Conjugation
APC-Cyanine7
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD73 positive B cells (red-filled) from CD19 negative CD73 negative Lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD73 positive B cells (red-filled) from CD19 negative CD73 negative Lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD19 (LT19) PE antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD19 (LT19) PE antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD73 (AD2) APC-Cy™7 antibody (4 ul reagent / 100 ul of peripheral whole blood).)
Related Product Information for anti-CD73 antibody
CD73 (ecto-5'-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
5'-nucleotidase
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
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Product Notes
The CD73 nt5e (Catalog #AAA128407) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD73 APC-Cyanine7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD73 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD73 nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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