Application Data
Mouse anti-Human CD79b Monoclonal Antibody | anti-CD79b antibody
anti-human CD79b-Purified Preservative Free
Gene Names
CD79B; B29; IGB; AGM6
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Purified Preservative Free
Synonyms
CD79b, Antibody; anti-human CD79b-Purified Preservative Free; Monoclonal anti-human CD79b(BCR-Igb); anti-CD79b antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
SN8/3A2-2E7
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
125
Applicable Applications for anti-CD79b antibody
WB (Western Blot), FCM/FACS (Flow Cytometry)
Performance
Five x 105 cultured Daudi human tumor cells were incubated 45 minutes on ice with 80 ul of anti-CD79b antibody at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC ( ), after which they were washed three times, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.50 log10 fluorescent units when compared to a Mouse IgG1 negative control ( ) at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Membrane preparation of human B prolymphocytic leukemia cells
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Application Data
Application Data
Related Product Information for anti-CD79b antibody
Information: Human CD79b is the B cell antigen receptor Igbeta chain (BCR-Igbeta) which associates with B cell antigen receptor Iga chain to form a functional heterodimer that interacts with membrane immunoglobulin (mIg). The mIgM component binds antigen and the Igalpha/Igbeta heterodimer transduces signals. Antibody SN8 recognizes the extracellular part of the BCR complex chain (CD79b) which has a molecular weight of approximately 38 kd
References
M. Okazaki, et al, (1993) Blood 81: 84-94. S. Vasile, et al, (1994) Mol Immunol 31: 419-427. A.L. DeFranco, et al, (1994) Chem Immunol 59: 156-172. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 667-675, 677-681. Chu PG, Arber DA. (2001) Appl Immunohistochem Molecul Morphol 9: 97-106.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,119 Da
NCBI Official Full Name
CD79b, partial
NCBI Official Synonym Full Names
CD79b molecule, immunoglobulin-associated beta
NCBI Official Symbol
CD79B
NCBI Official Synonym Symbols
B29; IGB; AGM6
NCBI Protein Information
B-cell antigen receptor complex-associated protein beta chain; Ig-beta; B-cell-specific glycoprotein B29; immunoglobulin-associated B29 protein; CD79b antigen (immunoglobulin-associated beta)
UniProt Protein Name
B-cell antigen receptor complex-associated protein beta chain
UniProt Gene Name
CD79B
UniProt Synonym Gene Names
B29; IGB
UniProt Entry Name
CD79B_HUMAN
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Product Notes
The CD79b cd79b (Catalog #AAA78181) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD79b-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD79b can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD79b cd79b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD79b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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