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WB (Western Blot)
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WB (Western Blot) (Detection of human CD79B by western blot. Samples: Whole cell lysate (50 ug) from Daudi, HeLa, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

CD79B recombinant antibody

Rabbit anti-CD79B Recombinant Monoclonal Antibody [BLR229K]

Gene Names
CD79B; B29; IGB; AGM6
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
CD79B; Monoclonal Recombinant Antibody; Rabbit anti-CD79B Recombinant Monoclonal Antibody [BLR229K]; CD antigen CD79b; immunoglobulin-associated B29 protein; Ig-beta; IGB; AGM6; CD79b antigen (immunoglobulin-associated beta); B-cell-specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; B29; CD79b molecule; immunoglobulin-associated beta; CD79B recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR229K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for CD79B recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 179 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD79B by western blot. Samples: Whole cell lysate (50 ug) from Daudi, HeLa, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human CD79B by western blot. Samples: Whole cell lysate (50 ug) from Daudi, HeLa, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of mouse CD79B by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CH27, CT26, WEHI-231, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of mouse CD79B by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CH27, CT26, WEHI-231, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of human CD79B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used for IP at 20 ul/mg lysate. CD79B was also immunoprecipitated by a second antibody against a different epitope of CD79B (Antibody 2). For blotting immunoprecipitated CD79B, AAA23876 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation) (Detection of human CD79B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876 lot 1) used for IP at 20 ul/mg lysate. CD79B was also immunoprecipitated by a second antibody against a different epitope of CD79B (Antibody 2). For blotting immunoprecipitated CD79B, AAA23876 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistchemistry)

(Detection of mouse CD79B by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistchemistry) (Detection of mouse CD79B by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human CD79B by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of human CD79B by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse CD79B by immunocytochemistry. Sample: FFPE section of CH27 cells. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse CD79B by immunocytochemistry. Sample: FFPE section of CH27 cells. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of human CD79B by immunocytochemistry. Sample: FFPE section of daudi cells. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human CD79B by immunocytochemistry. Sample: FFPE section of daudi cells. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human CD79B (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human CD79B (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of mouse CD79B (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse CD79B (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-CD79B recombinant monoclonal antibody (AAA23876) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for CD79B recombinant antibody
CD79B is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P40259].
Product Categories/Family for CD79B recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
974
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,119 Da
NCBI Official Full Name
B-cell antigen receptor complex-associated protein beta chain isoform 2
NCBI Official Synonym Full Names
CD79b molecule, immunoglobulin-associated beta
NCBI Official Symbol
CD79B
NCBI Official Synonym Symbols
B29; IGB; AGM6
NCBI Protein Information
B-cell antigen receptor complex-associated protein beta chain; Ig-beta; B-cell-specific glycoprotein B29; immunoglobulin-associated B29 protein; CD79b antigen (immunoglobulin-associated beta)
UniProt Protein Name
B-cell antigen receptor complex-associated protein beta chain
UniProt Gene Name
CD79B
UniProt Synonym Gene Names
B29; IGB
UniProt Entry Name
CD79B_HUMAN

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Product Notes

The CD79B cd79b (Catalog #AAA23876) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD79B Recombinant Monoclonal Antibody [BLR229K] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD79B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the CD79B cd79b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD79B, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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