FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-CD8alpha/Lyt-2 antibody YTS 169.4 Mouse lymphocytes were stained with an anti-CD44 control or the rabbit-chimeric version of YTS 169.4 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD8 alpha/Lyt-2 Recombinant Antibody | CD8 recombinant antibody
Anti-CD8 alpha/Lyt-2 [YTS 169.4]
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
CD8 alpha/Lyt-2, Recombinant Antibody; Anti-CD8 alpha/Lyt-2 [YTS 169.4]; Leu2; CD8; CD8A; CD8a molecule; CD8a antigen; Leu2 T-lymphocyte antigen; Lyt-2; Lyt2OKT8; p32T-cell antigen Leu2; T-cell antigen; T cell co-receptor; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; T8 T-cell antigen; CD8 recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
YTS 169.4
Specificity
Recognizes the murine CD8 cell surface antigen expressed by a subset of T lymphocytes. The antibody has been shown to show depleting activity in vivo.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
247
Applicable Applications for CD8 recombinant antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Antibody Type
Recombinant Antibody
Antibody Species
Rat
Original Species Name
Rat
Immunogen
Murine CD8 (lyt-2).
Chimeric Use Statement
This is a chimeric antibody created to reduce immunogenicity during in vivo applications.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-CD8alpha/Lyt-2 antibody YTS 169.4 Mouse lymphocytes were stained with an anti-CD44 control or the rabbit-chimeric version of YTS 169.4 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-CD8alpha/Lyt-2 antibody YTS 169.4 Mouse lymphocytes were stained with an anti-CD44 control or the rabbit-chimeric version of YTS 169.4 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD8 alpha/Lyt-2 antibody YTS 169.4 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTS 169.4 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD8 alpha/Lyt-2 antibody YTS 169.4 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTS 169.4 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Related Product Information for CD8 recombinant antibody
Recombinant monoclonal antibody to CD8 alpha/Lyt-2. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma YTS 169.4.
Product Categories/Family for CD8 recombinant antibody
References
Cobbold SP, Jayasuriya A, Nash A, Prospero TD, Waldmann H. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature. 1984 Dec 6-12;312(5994):548-51. PMID:6150440
Original Published Journal: Nature. 1984 Dec 6-12;312(5994):548-51.
Publication Note: Describes the production of the antibody and its effectivesness in CD8 subset depletion in vivo using flow cytometry studies.
Original Published Journal: Nature. 1984 Dec 6-12;312(5994):548-51.
Publication Note: Describes the production of the antibody and its effectivesness in CD8 subset depletion in vivo using flow cytometry studies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8 antigen, alpha chain
NCBI Official Symbol
Cd8a
NCBI Official Synonym Symbols
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Gene Name
Cd8a
UniProt Synonym Gene Names
Lyt-2; Lyt2
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Product Notes
The CD8 cd8a (Catalog #AAA72016) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD8 alpha/Lyt-2 [YTS 169.4] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 alpha/Lyt-2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD8 cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 alpha/Lyt-2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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