Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD8:FITC)
Rat CD8 Monoclonal Antibody | anti-CD8 antibody
RAT ANTI HUMAN CD8
Gene Names
CD8A; CD8; MAL; p32; Leu2
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD8, Antibody; RAT ANTI HUMAN CD8; CD8B1, MAL; anti-CD8 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
YTC182.20
Specificity
Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
Ig concentration 1.0mg/ml (varies by lot)
Sequence Length
235
Applicable Applications for anti-CD8 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Carrier
Yes
Immunogen
Mouse L cells transfected with human CD8 gene
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
Buffer Solution
Phosphate buffered saline
Target Species
Human
Species Cross-Reactivity
Reacts with: Cynomolgus monkey, Rhesus Monkey.
N.B. Antibody reactivity and working conditions may vary between species.
Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
N.B. Antibody reactivity and working conditions may vary between species.
Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
Preparation and Storage
It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD8:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD8:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD8)
Application Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD8)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8; MAL; p32; Leu2
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Gene Name
CD8A
UniProt Synonym Gene Names
MAL
UniProt Entry Name
CD8A_HUMAN
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Product Notes
The CD8 cd8a (Catalog #AAA49400) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD8 cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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