IHC (Immunohistochemistry)
(Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti- CD8 (Clone 5H10) (Cat# AAA71364) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rat anti-Mouse CD8 Monoclonal Antibody | anti-CD8 antibody
Rat anti CD8 (Ms) Monoclonal Antibody
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, ELISA, Western Blot
Purity
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
Synonyms
CD8, Antibody; Rat anti CD8 (Ms) Monoclonal Antibody; Cluster of Differentiation 8; anti-CD8 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
5H10
Specificity
This antibody recognizes mouse CD8 protein. The other species are not tested.
Purity/Purification
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-CD8 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antigen Preparation
A recombinant protein derived from extracellular domain (22-182aa) was used as an immunogen
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 24 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles
IHC (Immunohistochemistry)
(Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti- CD8 (Clone 5H10) (Cat# AAA71364) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohistochemistry)
(Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti- CD8 (Clone 5H10) (Cat# AAA71364) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-CD8 antibody
Background: The CD8 antigen is a cell surface glycoprotein found on majority of cytotoxic T lymphocytes, thymocytes and some peripheral blood T cells. It mediates efficient cell-cell interactions within the immune system. CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. Studies show that different antibodies against human CD8 exert opposite effects on MHC-peptide tetramer binding to the TCR: tetramer staining was enhanced by OKT8 antibody, while it was blocked with SK1 antibody. CD8a is a critical coreceptor of cytotoxic T cells.
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Product Notes
The CD8 (Catalog #AAA71364) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti CD8 (Ms) Monoclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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