Mouse CD86 Monoclonal Antibody | anti-CD86 antibody

MOUSE ANTI RAT CD86

Cat. Num. AAA12237

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD86, Antibody; MOUSE ANTI RAT CD86; anti-CD86 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 24F
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 323

• Applicable Applications for anti-CD86 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: HTLV-1 transformed Lewis-S1 cells; Fusion Partners
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Medium power)

Application Data

(Staining of rat splenocytes with Mouse anti Rat CD86:RPE)

Application Data

(Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?647 (AAA12237A647))

Application Data

(Staining of rat splenocytes with Mouse anti Rat CD86:Low Endotoxin)

Application Data

(Staining of rat splenocytes with Mouse anti Rat CD86:FITC)

Application Data

(Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?488 (AAA12237A488))

Application Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . High power)

Related Product Information for anti-CD86 antibody

Mouse anti Rat CD86 antibody, clone 24F specifically recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86.

NCBI and Uniprot Product Information

• NCBI GI #: 49456643
• NCBI GeneID: 942
• UniProt Accession #: P42081; Q13655; Q6FHB1; Q6GTS4; Q7M4L5; A0N0P0; B7Z2F3; B7Z702; E7ETN5; E9PC27
• Molecular Weight: 28,420 Da
• NCBI Official Full Name: CD86, partial
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86; B-lymphocyte activation antigen B7-2; BU63; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2
• UniProt Entry Name: CD86_HUMAN