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Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
Mouse CD86 Monoclonal Antibody | anti-CD86 antibody
MOUSE ANTI RAT CD86
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD86, Antibody; MOUSE ANTI RAT CD86; anti-CD86 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
24F
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
323
Applicable Applications for anti-CD86 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
HTLV-1 transformed Lewis-S1 cells
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Medium power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Medium power)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:RPE)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:RPE)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?647 (AAA12237A647))
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?647 (AAA12237A647))
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:Low Endotoxin)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:Low Endotoxin)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:FITC)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:FITC)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?488 (AAA12237A488))
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?488 (AAA12237A488))
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . High power)
Related Product Information for anti-CD86 antibody
Mouse anti Rat CD86 antibody, clone 24F specifically recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,420 Da
NCBI Official Full Name
CD86, partial
NCBI Official Synonym Full Names
CD86 molecule
NCBI Official Symbol
CD86
NCBI Official Synonym Symbols
B70; B7-2; B7.2; LAB72; CD28LG2
NCBI Protein Information
T-lymphocyte activation antigen CD86; B-lymphocyte activation antigen B7-2; BU63; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1
UniProt Protein Name
T-lymphocyte activation antigen CD86
UniProt Gene Name
CD86
UniProt Synonym Gene Names
CD28LG2
UniProt Entry Name
CD86_HUMAN
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Product Notes
The CD86 cd86 (Catalog #AAA12237) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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