Standard Curve (Sample)
Mouse CD86 ELISA Kit | CD86 elisa kit
Mouse CD86 ELISA Kit
Gene Names
Cd86; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
Reactivity
Mouse
Applications
ELISA
Synonyms
CD86; Mouse CD86 ELISA Kit; CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86 elisa kit
Reactivity
Mouse
Specificity
The Mouse CD86 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD86 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
309
Samples
Cell Culture Supernatants, Serum, Plasma.
Assay Type
Sandwich
Sensitivity
The minimum detectable dose of Mouse CD86 is typically less than 10 pg/ml.
Applicable Applications for CD86 elisa kit
Sandwich ELISA (SE)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD86 elisa kit
Background/Introduction: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Principle of the Assay: The Bioscience Mouse CD86 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD86 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD86 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD86 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD86 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD86 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Bioscience Mouse CD86 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD86 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD86 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD86 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD86 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD86 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,962 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD86
NCBI Official Synonym Full Names
CD86 antigen
NCBI Official Symbol
Cd86
NCBI Official Synonym Symbols
B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
NCBI Protein Information
T-lymphocyte activation antigen CD86; activation B7-2 antigen; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1
UniProt Protein Name
T-lymphocyte activation antigen CD86
UniProt Gene Name
Cd86
UniProt Synonym Gene Names
ETC-1
UniProt Entry Name
CD86_MOUSE
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Product Notes
The Mouse CD86 cd86 (Catalog #AAA17898) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17898 ELISA Kit recognizes Mouse CD86. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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