Application Data
(FCM staining of human PBMCs using CD86 MAb (BU63).)
Mouse CD86 Monoclonal Antibody | anti-CD86 antibody
CD86
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat.
Applications
Flow Cytometry, Functional Assay
Purity
Purified Ab conjugated to FITC
Synonyms
CD86, Antibody; CD86; Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2; anti-CD86 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
BU63
Specificity
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Purity/Purification
Purified Ab conjugated to FITC
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
217
Applicable Applications for anti-CD86 antibody
FCM/FACS (Flow Cytometry)
Localization
Cell surface
Positive Control
293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
Human Chromosomal Location
3q13.33
Immunogen
ARH-77 (B-lymphoblastoid cell line)
Quantity and Buffer
0.5 ml at 200ug/ml; Conjugated to FITC
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
Application Data
(FCM staining of human PBMCs using CD86 MAb (BU63).)
Application Data
(FCM staining of human PBMCs using CD86 MAb (BU63).)
Application Data
(Formalin-fixed, paraffin-embedded human esophageal tumor stained with CD86 MAb (BU63).)
Application Data
(Formalin-fixed, paraffin-embedded human esophageal tumor stained with CD86 MAb (BU63).)
References
1. Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF: The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994;84(5):1402-7.
2. Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994;180(5):1841-7.
3. Mauri D, Wyss-Coray T, Gallati H, Pichler WJ: Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol. 1995;155(1):118-27.
4. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
2. Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994;180(5):1841-7.
3. Mauri D, Wyss-Coray T, Gallati H, Pichler WJ: Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol. 1995;155(1):118-27.
4. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kDa
NCBI Official Full Name
T-lymphocyte activation antigen CD86 isoform 4
NCBI Official Synonym Full Names
CD86 molecule
NCBI Official Symbol
CD86
NCBI Official Synonym Symbols
B70; B7-2; B7.2; LAB72; CD28LG2
NCBI Protein Information
T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
UniProt Protein Name
T-lymphocyte activation antigen CD86
UniProt Gene Name
CD86
UniProt Synonym Gene Names
CD28LG2
UniProt Entry Name
CD86_HUMAN
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Product Notes
The CD86 cd86 (Catalog #AAA62572) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD86 reacts with Human, Mouse, Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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