CD86 recombinant antibody

Anti-CD86 [OX-48], Rabbit IgG, kappa

Cat. Num. AAA72467

• Gene Names: Cd86; B7-2
• Reactivity: Rat
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: CD86, Recombinant Antibody; Anti-CD86 [OX-48], Rabbit IgG, kappa; CD86; Anti-CD86 [OX-48]; B72; B-72; OX48; MRC OX-48; MRC OX48; CD86 recombinant antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: OX-48
• Specificity: OX-48 binds specifically to CD86 targets which are present on subsets of T and B blasts, spleen macrophages and polymorphs, peritoneal macrophages, dendritic cells. OX48 does not appear to bind the same epitope of CD86 as 24F (Stephens et al. 2004). CD86 is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes - expression is rapidly upregulated by B cell specific stimuli. CD86 acts as a ligand for CD28 and CTLA-4. CD86 works in tandem with CD80 to prime T cells. 2 splice isoforms exist.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for CD86 recombinant antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow-cytometryusingtheanti-CD86antibodyOX-48(AAA72467). Ratsplenocyteswerestainedwithanti-FluoresceinIgGantibody(4-4-20;isotypecontrol,shadedline)ortherabbitIgG1versionofOX-48(AAA72467,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-mouseIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)

IF (Immunofluorescence)

(Immunofluorescencestainingofratsplenocytesusinganti-CD86antibody(AAA72467)OX-48. ImmunofluorescenceanalysisofparaformaldehydefixedratsplenocytesimmobilizedonShi-fixcover-slipsandstainedwiththechimericrabbitIgGversionofOX-48(AAA72467)at10ug/mlfollowedbyAlexaFluor488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72467,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithanti-FluoresceinantibodyfollowedbyAlexaFluor488secondaryantibody.)

Related Product Information for CD86 recombinant antibody

OX-48 was prepared by immunizing mice with activated rat T cell blasts

References

Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol 24:1281 1987 N/A2828930
Note on publication:
Describes the use of Ox-48 among other anitbodies to identify new markers for activated T-cells, which might have expression related to theactive Vs quiescient state or may be involved in specific T-cell functions.

NCBI and Uniprot Product Information

• NCBI GI #: 81863176
• NCBI GeneID: 56822
• UniProt Accession #: Q62810
• Molecular Weight: 17,153 Da
• NCBI Official Full Name: B7-2
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: Cd86
• NCBI Official Synonym Symbols: B7-2
• NCBI Protein Information: T-lymphocyte activation antigen CD86; cd86 antigen; membrane glycoprotein
• UniProt Protein Name: B7-2
• UniProt Gene Name: Cd86
• UniProt Entry Name: Q62810_RAT