Application Data
Rat anti-Mouse CD8a (Ly 2) Monoclonal Antibody | anti-CD8a (Ly 2) antibody
Anti-Mouse CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Cytotoxicity Assay, Immunohistochemistry
Synonyms
CD8a (Ly 2), Antibody; Anti-Mouse CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b); CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b); Anti-Mouse CD8a (Ly 2) Monoclonal Antibody; anti-CD8a (Ly 2) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
YTS 169 AG 101 HL
Specificity
CD8a (Ly 2; Lyt 2) (Mouse CD8a (Ly 2))
Specifications:
Clone: YTS 169AG 101HL
Hybridoma Production:
Immunization: Immunogen: Mouse Ly-2 thymocytes
Donor: (Lou x DA) F1 rat
Fusion Partner: Myeloma Y3/Ag1.2.3
Specificity: Mouse CD8a (Ly 2)
Strains Tested: C57BL/6, BALB/c, AKR/J, C3H/He
Positive: C57BL/6, BALB/c, AKR/J, C3H/He
Negative: None
Specifications:
Clone: YTS 169AG 101HL
Hybridoma Production:
Immunization: Immunogen: Mouse Ly-2 thymocytes
Donor: (Lou x DA) F1 rat
Fusion Partner: Myeloma Y3/Ag1.2.3
Specificity: Mouse CD8a (Ly 2)
Strains Tested: C57BL/6, BALB/c, AKR/J, C3H/He
Positive: C57BL/6, BALB/c, AKR/J, C3H/He
Negative: None
Form/Format
Purified
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
235
Applicable Applications for anti-CD8a (Ly 2) antibody
FCM/FACS (Flow Cytometry), CA (Cytotoxic Assay), IHC (Immunohistochemistry)
Immunogen
Mouse Ly-2 thymocytes
Donor
(Lou x DA) F1 rat
Fusion Partner
myeloma Y3/Ag1.2.3
Presentation
Purified IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.
Preparation and Storage
Store Ascites at -20 degree C. For all other formats, store at 4 degree C. DO NOT FREEZE APC and PE conjugates. For long term storage (Purified, Biotin, FITC and No Azide), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Application Data
Application Data
Related Product Information for anti-CD8a (Ly 2) antibody
purified anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology1. It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro. This antibody has been reported to be suitable for use in immunohistochemistry on PFA-fixed paraffin-embedded tissue sections (antigen retrieval is required).7 This clone has been reported to work in immunohistochemistry6 (frozen sections).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,729 Da
NCBI Official Full Name
CD8a molecule
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8; MAL; p32; Leu2
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Gene Name
CD8A
UniProt Synonym Gene Names
MAL
UniProt Entry Name
CD8A_HUMAN
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Product Notes
The CD8a (Ly 2) cd8a (Catalog #AAA74165) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8a (Ly 2) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), CA (Cytotoxic Assay), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD8a (Ly 2) cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8a (Ly 2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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