Rabbit anti-Human CD97 Monoclonal Antibody | anti-ADGRE5 antibody

Recombinant Anti-CD97 Antibody, Rabbit Monoclonal

Cat. Num. AAA255388

• Gene Names: ADGRE5; CD97; TM7LN1
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A
• Synonyms: CD97, Antibody; Recombinant Anti-CD97 Antibody, Rabbit Monoclonal; CD97 Antibody, Rabbit MAb; adhesion G protein-coupled receptor E5; Anti-CD97 Antibody; Anti-TM7LN1 Antibody; anti-ADGRE5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 024
• Specificity: Human CD97.; No cross-reactivity in ELISA with Human CD55/DAF
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ADGRE5 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD97 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD97 (rh CD97).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human CD97 expression on K562 cells. Cells were stained with purified anti-Human CD97, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human CD97 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD97 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membranes.)

Related Product Information for anti-ADGRE5 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium-binding site. Mature CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adhere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isoforms that are related to the calcium-binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.

References

Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Gray JX, et al. (1996) CD97 is a processed, seven-transmembrane, heterodimeric receptor associated with inflammation. The journal of Immunology. 157 (12): 5438-47.Hamann J, et al. (1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.European Journal of Immunology. 28 (5): 1701-7.

NCBI and Uniprot Product Information

• NCBI GI #: 17978489
• NCBI GeneID: 976
• NCBI Accession #: NP_001775.2
• NCBI GenBank Nucleotide #: NM_001784.4
• UniProt Accession #: P48960; O00718; O76101; Q8NG72; Q8TBQ7; A8K7Z4; B2RBJ9
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: CD97 antigen isoform 2 preproprotein
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor E5
• NCBI Official Symbol: ADGRE5
• NCBI Official Synonym Symbols: CD97; TM7LN1
• NCBI Protein Information: CD97 antigen
• UniProt Protein Name: CD97 antigen
• UniProt Gene Name: CD97
• UniProt Entry Name: CD97_HUMAN