Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-CD99 (CM0261) for ELISA capture of human recombinant CD99 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Mouse CD99 Monoclonal Antibody | anti-CD99 antibody
CD99 (Extracellular region) M026 Antibody
Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human, Rat, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
CD99, Antibody; CD99 (Extracellular region) M026 Antibody; CD99 (Extracellular region); CD099 M026; anti-CD99 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M026
Specificity
Clone M026 detects 30 and 32kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CD99 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone M026 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year. The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20 degree C upon arrival for long term storage.
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-CD99 (CM0261) for ELISA capture of human recombinant CD99 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-CD99 (CM0261) for ELISA capture of human recombinant CD99 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M026). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M026). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot analysis of human cell lysates: A549 (lane 1), A431 (lane 2), LNCaP (lane 3), MDA-MB-231 (lane 4) and MeWo (lane 5). The blot was probed with mouse monoclonal anti-CD99 (CM0261) at 1:1000.)
WB (Western Blot)
(Western blot analysis of human cell lysates: A549 (lane 1), A431 (lane 2), LNCaP (lane 3), MDA-MB-231 (lane 4) and MeWo (lane 5). The blot was probed with mouse monoclonal anti-CD99 (CM0261) at 1:1000.)
Related Product Information for anti-CD99 antibody
The glycosylated transmembrane protein CD99 is involved in many essential cellular functions including cell adhesion, migration, cell death, differentiation, and intracellular protein trafficking. The CD99 gene encodes two distinct proteins, type I is 32kDa and type II is 28kDa, which are a result of the alternative splicing of the cytoplasmic region. These CD99 isoforms are expressed in a cell-type-specific manner and may have distinct functions. CD99 is overexpressed in several types of sarcomas, lymphomas, gliomas, neuroendocrine tumors, and some breast cancers. In these tumors, CD99 may have oncogenetic functions that promote migration, invasion, and metastasis of tumor cells. However, other neoplasms, carcinomas, and sarcomas have CD99 expression in benign or early-stage tumors, but lower expression in the advanced-stage counterparts. In these tumors, CD99 may have oncosuppressor signaling, and its re-expression can lead to the reversal of malignancy. Thus, CD99 is an important membrane protein involved in many aspects of cell migration and adhesion in normal and diseased cells.
References
Manara MC, et al. (2018) Genes (Basel). 9(3) pii: E159.
Pasello M, et al. (2018) J Cell Commun Signal. 12(1):55.
Pasello M, et al. (2018) J Cell Commun Signal. 12(1):55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,848 Da
NCBI Official Full Name
CD99 antigen isoform a
NCBI Official Synonym Full Names
CD99 molecule
NCBI Official Symbol
CD99
NCBI Official Synonym Symbols
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
NCBI Protein Information
CD99 antigen; E2 antigen; surface antigen MIC2; T-cell surface glycoprotein E2; MIC2 (monoclonal antibody 12E7); antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13
UniProt Protein Name
CD99 antigen
UniProt Gene Name
CD99
UniProt Synonym Gene Names
MIC2; MIC2X; MIC2Y
UniProt Entry Name
CD99_HUMAN
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Product Notes
The CD99 cd99 (Catalog #AAA71581) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD99 (Extracellular region) M026 Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD99 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD99 cd99 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD99, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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