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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit CDC34 Monoclonal Antibody | anti-CDC34 antibody
CDC34 Antibody
Gene Names
CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
CDC34, Antibody; CDC34 Antibody; Cdc 34 antibody; Cdc34 antibody; Cell division cycle 34 antibody; Cell division cycle 34 homolog (S. cerevisiae) antibody; Cell division cycle 34 homolog antibody; E2 CDC34 antibody; UB2R1_HUMAN antibody; UBC 3 antibody; UBC3 antibody; UBCH3 antibody; UBE2 R1 antibody; UBE2R1 antibody; Ubiquitin carrier protein antibody; Ubiquitin conjugating enzyme Cdc34 antibody; Ubiquitin conjugating enzyme E2 32 kDa complementing antibody; Ubiquitin protein ligase antibody; Ubiquitin protein ligase R1 antibody; Ubiquitin-conjugating enzyme E2 R1 antibody; Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody; Ubiquitin-conjugating enzyme E2-CDC34 antibody; Ubiquitin-protein ligase R1 antibody; anti-CDC34 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JJ086-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CDC34 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat)
WB (Western Blot)
(Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat)
Related Product Information for anti-CDC34 antibody
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
Product Categories/Family for anti-CDC34 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 R1
NCBI Official Synonym Full Names
cell division cycle 34, ubiqiutin conjugating enzyme
NCBI Official Symbol
CDC34
NCBI Official Synonym Symbols
UBC3; UBCH3; UBE2R1; E2-CDC34
NCBI Protein Information
ubiquitin-conjugating enzyme E2 R1
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 R1
UniProt Gene Name
CDC34
UniProt Synonym Gene Names
UBCH3; UBE2R1
UniProt Entry Name
UB2R1_HUMAN
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Product Notes
The CDC34 cdc34 (Catalog #AAA312363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC34 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC34 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDC34 cdc34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC34, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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