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WB (Western Blot)
(Detection of human CDK7 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, Jurkat, U2OS, LNCaP, RKO, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 .)
CDK7 recombinant antibody
Rabbit anti-CDK7 Recombinant Monoclonal Antibody [BL-80-5D4]
Gene Names
CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CDK7, Recombinant Antibody; Rabbit anti-CDK7 Recombinant Monoclonal Antibody [BL-80-5D4]; serine/threonine kinase stk1; TFIIH basal transcription factor complex kinase subunit; STK1; Serine/threonine-protein kinase 1; serine/threonine protein kinase 1; p39MO15; p39 Mo15; MO15; kinase subunit of CAK; 39 KDa protein kinase; HCAK; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); cyclin-dependent kinase 7; cell division protein kinase 7; CDKN7; CDK-activating kinase 1; CAK1; CAK; homolog of Xenopus MO15 Cdk-activating kinase; serine/threonine protein kinase MO15; Cyclin-dependent kinase 7; cyclin dependent kinase 7; CDK7 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BL-80-5D4
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
40ug/ml (varies by lot)
Applicable Applications for CDK7 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 296 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CDK7 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, Jurkat, U2OS, LNCaP, RKO, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 .)
WB (Western Blot)
(Detection of human CDK7 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, Jurkat, U2OS, LNCaP, RKO, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 .)
IP (Immunoprecipitation)
(Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2) used for IP at 20 ul/mg lysate. CDK7 was also immunoprecipitated by a previous lot of this antibody (lot AAA213531-1) and rabbit anti-CDK7 antibody For blotting immunoprecipitated CDK7, AAA213531 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2) used for IP at 20 ul/mg lysate. CDK7 was also immunoprecipitated by a previous lot of this antibody (lot AAA213531-1) and rabbit anti-CDK7 antibody For blotting immunoprecipitated CDK7, AAA213531 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human CDK7 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human CDK7 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CDK7 in FFPE MCF7 cells by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CDK7 in FFPE MCF7 cells by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human CDK7 by flow cytometry. Sample: MOLT4 cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CDK7 by flow cytometry. Sample: MOLT4 cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (AAA213531 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for CDK7 recombinant antibody
Cyclin-dependent kinase 7 (CDK7) is a member of the CDK family of protein kinases that regulate cell cycle progression. CDK7 is the catalytic subunit in a trimeric complex with cyclin H and MAT1 that functions as a Cdk-activating kinase (CAK). CDK7 activates the kinase activities of CDK1, CDK2, CDK4, and CDK6 by threonine phosphorylation. In addition to its cell cycle function CDK7 has also been found to be an essential component of the transcription factor TFIIH. Several substrates of CDK7 are involved in transcription, further indicating a role in transcription.
Product Categories/Family for CDK7 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,038 Da
NCBI Official Full Name
cyclin-dependent kinase 7
NCBI Official Synonym Full Names
cyclin-dependent kinase 7
NCBI Official Symbol
CDK7
NCBI Official Synonym Symbols
CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
NCBI Protein Information
cyclin-dependent kinase 7; CAK; p39 Mo15; protein kinase; 39 KDa protein kinase; kinase subunit of CAK; CDK-activating kinase 1; serine/threonine kinase stk1; cell division protein kinase 7; serine/threonine protein kinase 1; serine/threonine-protein kina
UniProt Protein Name
Cyclin-dependent kinase 7
UniProt Gene Name
CDK7
UniProt Synonym Gene Names
CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15
UniProt Entry Name
CDK7_HUMAN
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Product Notes
The CDK7 cdk7 (Catalog #AAA213531) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CDK7 Recombinant Monoclonal Antibody [BL-80-5D4] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK7 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CDK7 cdk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK7, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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