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product-image-AAA30806_IF8.png IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.Amyloid-? Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)

Mouse CDX2 Monoclonal Antibody | anti-CDX2 antibody

CDX2 Monoclonal Antibody (14H6)

Gene Names
CDX2; CDX3; CDX-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
CDX2, Antibody; CDX2 Monoclonal Antibody (14H6); Homeobox protein CDX-2; CDX2; CDX3; CDX-3; Caudal-type homeobox protein 2; anti-CDX2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
14H6
Specificity
The antibody detects endogenous CDX2 proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-CDX2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000
IHC: 1:200
IF: 1:200
Immunogen
Synthetic Peptide of CDX2
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.Amyloid-? Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)

product-image-AAA30806_IF8.png IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.Amyloid-? Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

product-image-AAA30806_IHC7.png IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

product-image-AAA30806_IHC6.png IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

product-image-AAA30806_IHC5.png IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.CDX2 Monoclonal Antibody(14H6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(IHC staining of human rectal cancer tissue. diluted at 1:200.)

product-image-AAA30806_IHC4.png IHC (Immunohistochemistry) (IHC staining of human rectal cancer tissue. diluted at 1:200.)

Application Data

(Liu. Huiming. et al. "The role of CDX2 in renal tubular lesions during diabetic kidney disease." Aging (Albany NY) 13.5 (2021): 6782.)

product-image-AAA30806_APP3.png Application Data (Liu. Huiming. et al. "The role of CDX2 in renal tubular lesions during diabetic kidney disease." Aging (Albany NY) 13.5 (2021): 6782.)

Application Data

(Liu. Huiming. et al. "The role of CDX2 in renal tubular lesions during diabetic kidney disease." Aging (Albany NY) 13.5 (2021): 6782.)

product-image-AAA30806_APP2.png Application Data (Liu. Huiming. et al. "The role of CDX2 in renal tubular lesions during diabetic kidney disease." Aging (Albany NY) 13.5 (2021): 6782.)

WB (Western Blot)

(Western blot analysis of 1) 293T. 2) Mouse Heart tissue. diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)

product-image-AAA30806_WB.png WB (Western Blot) (Western blot analysis of 1) 293T. 2) Mouse Heart tissue. diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
Related Product Information for anti-CDX2 antibody
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]
Product Categories/Family for anti-CDX2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,520 Da
NCBI Official Full Name
homeobox protein CDX-2
NCBI Official Synonym Full Names
caudal type homeobox 2
NCBI Official Symbol
CDX2
NCBI Official Synonym Symbols
CDX3; CDX-3
NCBI Protein Information
homeobox protein CDX-2; caudal-type homeobox protein 2; caudal type homeobox transcription factor 2; caudal type homeo box transcription factor 2
UniProt Protein Name
Homeobox protein CDX-2
UniProt Gene Name
CDX2
UniProt Synonym Gene Names
CDX3
UniProt Entry Name
CDX2_HUMAN

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Product Notes

The CDX2 cdx2 (Catalog #AAA30806) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CDX2 Monoclonal Antibody (14H6) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000 IHC: 1:200 IF: 1:200. Researchers should empirically determine the suitability of the CDX2 cdx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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