Application Data
(Fig. 2: Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).)
Mouse anti-Human CEACAM5 Monoclonal Antibody | anti-CEACAM5 antibody
anti-human CEACAM5 monoclonal antibody
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human
Applications
Western Blot, ELISA, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CEACAM5, Antibody; anti-human CEACAM5 monoclonal antibody; CD66e; CEA; anti-CEACAM5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1/kappa
Clone Number
26/3/13
Specificity
anti-human CEACAM5 (CEA; CD66e)
Purity/Purification
Protein G
Form/Format
Phosphate buffered saline, pH 7.2
Sequence Length
702
Applicable Applications for anti-CEACAM5 antibody
WB (Western Blot), ELISA, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Immunogen
Immunisation with extracted protein of CEACAM5
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Preparation and Storage
Short term: 2°C - 8°C; long term: -20°C (avoid repeated freezing and thawing)
Application Data
(Fig. 2: Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).)
Application Data
(Fig. 2: Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).)
Application Data
(Fig.1: FC analysis of BOSC23 cells using 26/3/13 Cat.# AAA71463. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/3/13 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.)
Application Data
(Fig.1: FC analysis of BOSC23 cells using 26/3/13 Cat.# AAA71463. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/3/13 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.)
Related Product Information for anti-CEACAM5 antibody
Description
Purified monoclonal mouse antibody
Background
CEACAM5(CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein with a Mrof 180,000-200,000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas (3). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).
Purified monoclonal mouse antibody
Background
CEACAM5(CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein with a Mrof 180,000-200,000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas (3). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).
References
Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
Grunert F, Stocks SC, Nagel G., Zimmermann W, Thompson JA, Jantscheff P and Kromer B. (1996). CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. In Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto et al., eds.), Garland Publishing Inc., New York and London, pp. 1012-1025.
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
Grunert F, Stocks SC, Nagel G., Zimmermann W, Thompson JA, Jantscheff P and Kromer B. (1996). CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. In Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto et al., eds.), Garland Publishing Inc., New York and London, pp. 1012-1025.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
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Product Notes
The CEACAM5 ceacam5 (Catalog #AAA71463) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM5 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CEACAM5 ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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