IF (Immunofluorescence)
(Immunofluorescence staining of Human CEACAM6 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CEACAM6 monoclonal antibody (1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Rabbit anti-Human CEACAM6 Monoclonal Antibody | anti-CEACAM6 antibody
Recombinant Anti-CEACAM6 Antibody, Rabbit Monoclonal
Gene Names
CEACAM6; NCA; CEAL; CD66c
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Protein A
Synonyms
CEACAM6, Antibody; Recombinant Anti-CEACAM6 Antibody, Rabbit Monoclonal; CEACAM6/CD66c Antibody, Rabbit MAb; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); Anti-CD66c Antibody; Anti-CEA Antibody; Anti-CEAL Antibody; Anti-NCA Antibody; anti-CEACAM6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
408
Specificity
Human CEACAM6/CD66c.
No cross-reactivity in ELISA with Human CEACAM1/CD66a, Human CEACAM5/CD66e
No cross-reactivity in ELISA with Human CEACAM1/CD66a, Human CEACAM5/CD66e
Purity/Purification
Protein A
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-CEACAM6 antibody
FCM/FACS (Flow Cytometry), ELISA
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD66c protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM6 (rhCEACAM6).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of Human CEACAM6 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CEACAM6 monoclonal antibody (1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
IF (Immunofluorescence)
(Immunofluorescence staining of Human CEACAM6 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CEACAM6 monoclonal antibody (1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CEACAM6 expression on human peripheral blood granulocytes. Cells were stained with purified anti-Human CEACAM6, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human CEACAM6 expression on human peripheral blood granulocytes. Cells were stained with purified anti-Human CEACAM6, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.)
References
Duxbury MS, et al. (2004) Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness. Oncogene. 23(34): 5834-42.Lewis-Wambi JS, et al. (2008) Overexpression of CEACAM6 promotes migration and invasion of oestrogen-deprived breast cancer cells. Eur J Cancer. 44(12): 1770-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 6 preproprotein
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 6
NCBI Official Symbol
CEACAM6
NCBI Official Synonym Symbols
NCA; CEAL; CD66c
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 6
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 6
UniProt Gene Name
CEACAM6
UniProt Synonym Gene Names
NCA
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Product Notes
The CEACAM6 ceacam6 (Catalog #AAA255204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CEACAM6 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CEACAM6 ceacam6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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