IHC (Immunohistochemisry)
(Immunohistochemical analysis of parafin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX)
Rabbit Choline Acetyltransferase Monoclonal Antibody | anti-CHAT antibody
Choline Acetyltransferase
Gene Names
CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Protein A affinity purified.
Synonyms
Choline Acetyltransferase, Antibody; Choline Acetyltransferase; CHOACTase; ChAT; Choline acetylase; CHAT; Choline O-acetyltransferase; anti-CHAT antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
JA67-11
Purity/Purification
Protein A affinity purified.
Form/Format
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Sequence Length
630
Applicable Applications for anti-CHAT antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot)
Conjugation
AF647
Immunogen
Synthetic peptide within Human Choline Acetyltransferase aa 699-748 / 748.
Positive control
Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela, N2A, SH SY5Y, mouse brain tissue, rat stomach tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.
Subcellular location
Cytosol, nucleus, cytoplasm, neuron projection, presynapse
Preparation and Storage
Store at 4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze / thaw cycles
IHC (Immunohistochemisry)
(Immunohistochemical analysis of parafin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of parafin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX)
WB (Western Blot)
(Western blot analysis of Choline Acetyltransferase on di ferent lysates withRabbit anti-Choline Acetyltransferase antibody at 1/5,000 dilution.Lane 1: Human brain tissue lysate.Lane 2: Mouse brain tissue lysate.Lane 3: Rat brain tissue lysate. Lysates/proteins at 20 ug/Lane.Predicted band size: 83 kDa.Observed band size: 60-83 kDa.Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4 degree C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
WB (Western Blot)
(Western blot analysis of Choline Acetyltransferase on di ferent lysates withRabbit anti-Choline Acetyltransferase antibody at 1/5,000 dilution.Lane 1: Human brain tissue lysate.Lane 2: Mouse brain tissue lysate.Lane 3: Rat brain tissue lysate. Lysates/proteins at 20 ug/Lane.Predicted band size: 83 kDa.Observed band size: 60-83 kDa.Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4 degree C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody AAA268423 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody AAA268423 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-CHAT antibody
Choline acetyltransferase (also designated choactase, choline O-acetyltransferase) synthesizes acetylcholine in cholinergic neurons. Multiple choactase mRNAs with di ferent 5'noncoding regions are expressed as R-, N1, N2-, S- and M-types. N1-, N2- and R-type mRNAs produce a single short enzyme, while M-type mRNA produces both long and short enzymes. The long enzyme is targeted to the nuclei of ce ls, whereas the short protein is found in cytoplasm. A novel NFkB binding site is located within the nerve growth factor responsive enhancer element that is recognized by the NFkB protein p49, but not p65 or p50. Decreased choactase expression and increased NFkB activity are associated with aging and Alzheimer's disease, indicating that p49 is a negative regulator of choactase expression and suggesting a possible mechanism for aging-associated declines in cholinergic function. Phosphorylation of choactase has been shown to enhance choactase catalytic activity. Specifica ly, Serine 440 is found to be the phosphorylation site in a recombinant human short choactase by protein kinase C and is involved in regulation of the enzyme catalytic activity and binding to subce lular membranes
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted band size: 83 kDa
NCBI Official Full Name
choline O-acetyltransferase isoform 1
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
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Product Notes
The CHAT chat (Catalog #AAA268423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Choline Acetyltransferase reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Choline Acetyltransferase can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CHAT chat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Choline Acetyltransferase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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