IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver tissue. 1, CK7 Monoclonal Antibody (12D7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (Room Temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B)
Mouse CK7 Monoclonal Antibody | anti-CK7 antibody
CK7 Mouse Monoclonal Antibody
Gene Names
KRT7; K7; CK7; SCL; K2C7
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification using immunogen.
Synonyms
CK7, Antibody; CK7 Mouse Monoclonal Antibody; CK-7; CK7; cytokeratin 7; Cytokeratin-7; K2C7; K7; keratin 7; anti-CK7 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
12D7
Specificity
The CK7 Mouse Monoclonal antibody detects endogenous CK7 proteins.
Purity/Purification
Affinity purification using immunogen.
Form/Format
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
469
Applicable Applications for anti-CK7 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Target Name
CK7
Preparation and Storage
Store at -20°C
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver tissue. 1, CK7 Monoclonal Antibody (12D7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (Room Temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver tissue. 1, CK7 Monoclonal Antibody (12D7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (Room Temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohiostchemistry)
(IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200.)
IHC (Immunohiostchemistry)
(IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200.)
WB (Western Blot)
(Western blot analysis of Hela (lane 1), Mouse Kidney (lane 2) and Mouse Brain (lane 3), using diluted at 1:2,000.)
WB (Western Blot)
(Western blot analysis of Hela (lane 1), Mouse Kidney (lane 2) and Mouse Brain (lane 3), using diluted at 1:2,000.)
Related Product Information for anti-CK7 antibody
Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.
Product Categories/Family for anti-CK7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
SDS-PAGE MW: 54kd
NCBI Official Full Name
keratin, type II cytoskeletal 7
NCBI Official Synonym Full Names
keratin 7, type II
NCBI Official Symbol
KRT7
NCBI Official Synonym Symbols
K7; CK7; SCL; K2C7
NCBI Protein Information
keratin, type II cytoskeletal 7
UniProt Protein Name
Keratin, type II cytoskeletal 7
UniProt Gene Name
KRT7
UniProt Synonym Gene Names
SCL; CK-7; K7
UniProt Entry Name
K2C7_HUMAN
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Product Notes
The CK7 krt7 (Catalog #AAA300702) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CK7 Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CK7 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CK7 krt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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