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WB (Western Blot)
(Detection of human CK7 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HCT 116, OVCAR-3, OVCAR-4, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
CK7 recombinant antibody
Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]
Gene Names
KRT7; K7; CK7; SCL; K2C7
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CK7, Recombinant Antibody; Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]; keratin 7, type II; type II mesothelial keratin K7; SCL; sarcolectin; Keratin-7; keratin, type II cytoskeletal 7; type-II keratin Kb7; K7; K2C7; Cytokeratin-7; cytokeratin 7; CK-7; CK7; keratin, simple epithelial type I, K7; keratin, 55K type II cytoskeletal; Keratin, type II cytoskeletal 7; keratin 7; CK7 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BC1
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for CK7 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
C-terminal peptide
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CK7 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HCT 116, OVCAR-3, OVCAR-4, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CK7 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HCT 116, OVCAR-3, OVCAR-4, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used for IP at 20 ul/mg lysate. CK7 was also immunoprecipitated by another antibody against a different epitope of CK7. For blotting immunoprecipitated CK7, AAA213632 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human CK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632 lot 1) used for IP at 20 ul/mg lysate. CK7 was also immunoprecipitated by another antibody against a different epitope of CK7. For blotting immunoprecipitated CK7, AAA213632 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemisry)
(Detection of human CK7 in breast carcinoma by IHC. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody (AAA213632 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohistochemisry)
(Detection of human CK7 in breast carcinoma by IHC. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody (AAA213632 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry)
(Detection of human CK7 in NCI-H1975 cells by ICC. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody (AAA213632 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry)
(Detection of human CK7 in NCI-H1975 cells by ICC. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody (AAA213632 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
FCM/FACS (Flow Cytometry)
(Detection of human CK7 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CK7 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CK7 recombinant monoclonal antibody [BC1] (AAA213632) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for CK7 recombinant antibody
Cytokeratin 7 is a type II member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK7 is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. [taken from NCBI Entrez Gene (Gene ID: 3855)].
Product Categories/Family for CK7 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,386 Da
NCBI Official Full Name
keratin, type II cytoskeletal 7
NCBI Official Synonym Full Names
keratin 7
NCBI Official Symbol
KRT7
NCBI Official Synonym Symbols
K7; CK7; SCL; K2C7
NCBI Protein Information
keratin, type II cytoskeletal 7; CK-7; keratin-7; sarcolectin; cytokeratin 7; cytokeratin-7; type-II keratin Kb7; type II mesothelial keratin K7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7
UniProt Protein Name
Keratin, type II cytoskeletal 7
UniProt Gene Name
KRT7
UniProt Synonym Gene Names
SCL; CK-7; K7
UniProt Entry Name
K2C7_HUMAN
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Product Notes
The CK7 krt7 (Catalog #AAA213632) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CK7 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CK7 krt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK7, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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