IHC (Immunohistochemistry)
(Immunohistochemistry: Human colon carcinoma (FFPE) stained with Mouse anti-Claudin-5 (Cat# 605-630) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Mouse anti-Human Claudin 5 Monoclonal Antibody
Mouse anti Claudin 5 Monoclonal Antibody
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot, Immunohistochemistry
Purity
Protein A-Affinity Chromatography according to Isotyping
Synonyms
Claudin 5, Antibody; Mouse anti Claudin 5 Monoclonal Antibody; anti-Claudin 5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4C3C2
Specificity
This antibody recognizes human Claudin 5 protein. The other species are not tested.
Purity/Purification
Protein A-Affinity Chromatography according to Isotyping
Form/Format
Supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-Claudin 5 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Preparation
A synthetic peptide of human Claudin 5
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 24 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry: Human colon carcinoma (FFPE) stained with Mouse anti-Claudin-5 (Cat# 605-630) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohistochemistry)
(Immunohistochemistry: Human colon carcinoma (FFPE) stained with Mouse anti-Claudin-5 (Cat# 605-630) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-Claudin 5 antibody
Claudin 5 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. CLDN5 affects lncRNAs acting as ceRNA dynamics contributing to regulating blood brain barrier permeability in tumor brain metastasis. Among other tissues, Claudin-5 was observed to localize to cardiomyocytes, endothelial cells, and a subset of fibroblasts in non-failing human heart sections. It is transcriptionally downregulated resulting in dramatically reduced protein levels in human heart failure. It was recently shown that strong claudin 5 expression is a poor prognostic sign in pancreatic adenocarcinoma.
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Product Notes
The Claudin 5 (Catalog #AAA71368) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Claudin 5 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Claudin 5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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