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product-image-AAA328872_IHC8.jpg IHC (Immunohistochemistry) (AAA328872 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

Mouse CLDN6 Monoclonal Antibody | anti-CLDN6 antibody

CLDN6 Mouse mAb

Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Synonyms
CLDN6, Antibody; CLDN6 Mouse mAb; Claudin-6; Claudin6; CLD6_HUMAN; CLDN 6; CLDN6; OTTHUMP00000159248; Skullin 2; Skullin; UNQ757/PRO1488; anti-CLDN6 antibody
Ordering
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AFB17981
Specificity
Affinity-chromatography.
Form/Format
Liquid. Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
220
Applicable Applications for anti-CLDN6 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Fragment
Fab fragment
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(AAA328872 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

product-image-AAA328872_IHC8.jpg IHC (Immunohistochemistry) (AAA328872 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(AAA328872 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

product-image-AAA328872_IHC10.jpg IHC (Immunohistochemistry) (AAA328872 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(AAA328872 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

product-image-AAA328872_IHC11.jpg IHC (Immunohistochemisry) (AAA328872 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(AAA328872 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

product-image-AAA328872_IHC13.jpg IHC (Immunohiostchemistry) (AAA328872 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from HepG2 cells, using CLDN6 Mouse mAb .The lane on the left was treated with blocking peptide.)

product-image-AAA328872_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using CLDN6 Mouse mAb .The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CLDN6 antibody
Function: Plays a major role in tight junction-specific obliteration of the intercellular space.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Subcellular Location: Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Tissue Specificity: Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

Subunit Structure: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with CLDN1, CD81 and OCLN (PubMed:20375010).

Similarity: Belongs to the claudin family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)24kD
Predicted Molecular Weight: (Calculated)23kDa
NCBI Official Full Name
claudin-6
NCBI Official Synonym Full Names
claudin 6
NCBI Official Symbol
CLDN6
NCBI Protein Information
claudin-6
UniProt Protein Name
Claudin-6
UniProt Gene Name
CLDN6
UniProt Entry Name
CLD6_HUMAN

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Product Notes

The CLDN6 cldn6 (Catalog #AAA328872) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CLDN6 Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CLDN6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CLDN6 cldn6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLDN6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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