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IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue. 1.Cleaved PARP Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Mouse anti-Human Cleaved PARP Monoclonal Antibody | anti-PARP antibody
Cleaved PARP Monoclonal Antibody (Mix)
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Cleaved PARP, Antibody; Cleaved PARP Monoclonal Antibody (Mix); Poly [ADP-ribose] polymerase 1; PARP1; ADPRT; PPOL; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; anti-PARP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
Mix
Specificity
The antibody detects endogenous pro and active PARP protein.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-PARP antibody
IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
Synthetic Peptide of Cleaved PARP
Modification
Cleaved Specific
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue. 1.Cleaved PARP Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue. 1.Cleaved PARP Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cell. 1.BRCA1 Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cell. 1.BRCA1 Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.Cleaved PARP Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.Cleaved PARP Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot)
(Western blot analysis of Jurkat. diluted at 1:3000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
WB (Western Blot)
(Western blot analysis of Jurkat. diluted at 1:3000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
Related Product Information for anti-PARP antibody
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-PARP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113,084 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase 1
NCBI Official Symbol
PARP1
NCBI Official Synonym Symbols
PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
NCBI Protein Information
poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-
UniProt Protein Name
Poly [ADP-ribose] polymerase 1
UniProt Gene Name
PARP1
UniProt Synonym Gene Names
ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
UniProt Entry Name
PARP1_HUMAN
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Product Notes
The PARP parp1 (Catalog #AAA320447) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cleaved PARP Monoclonal Antibody (Mix) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cleaved PARP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PARP parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cleaved PARP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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