IHC (Immunohiostchemistry)
(Fig.2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Mouse Collagen I Monoclonal Antibody | anti-COL1A1 antibody
Collagen I Mouse Monoclonal Antibody (4H10)
Gene Names
COL1A1; OI1; OI2; OI3; OI4; EDSC; EDSARTH1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Collagen I, Antibody; Collagen I Mouse Monoclonal Antibody (4H10); Mouse Anti-Collagen I Mouse Monoclonal Antibody (4H10); Collagen alpha-1(I) chain; Alpha-1 type I collagen; anti-COL1A1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Specificity
The antibody detects endogenous Collagen I protein.
Purity/Purification
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1464
Applicable Applications for anti-COL1A1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
Synthetic Peptide of Collagen I
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Fig.2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry)
(Fig.2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Fig.1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Fig.1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue. 1, Collagen I Mouse Monoclonal Antibody (4H10) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Related Product Information for anti-COL1A1 antibody
COL1A1 (collagen type I alpha 1 chain) encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for COL1A1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Collagen alpha-1(I) chain
NCBI Official Synonym Full Names
collagen type I alpha 1 chain
NCBI Official Symbol
COL1A1
NCBI Official Synonym Symbols
OI1; OI2; OI3; OI4; EDSC; EDSARTH1
NCBI Protein Information
collagen alpha-1(I) chain
UniProt Protein Name
Collagen alpha-1(I) chain
UniProt Gene Name
COL1A1
UniProt Entry Name
CO1A1_HUMAN
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Product Notes
The COL1A1 col1a1 (Catalog #AAA330127) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Collagen I Mouse Monoclonal Antibody (4H10) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen I can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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