IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).)
Mouse Collagen IV (COL4A1/COL4A2) Monoclonal Antibody | anti-COL4A1 antibody
Collagen IV (COL4A1/COL4A2) MouseMonoclonal Antibody [Clone M3F7]
Gene Names
COL4A1; ICH; HANAC; POREN1; arresten
Reactivity
Human, Rat and Quail
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Collagen IV (COL4A1/COL4A2), Antibody; Collagen IV (COL4A1/COL4A2) MouseMonoclonal Antibody [Clone M3F7]; Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Type IV Alpha 2; RATOR; anti-COL4A1 antibody
Host
Mouse
Reactivity
Human, Rat and Quail
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
M3F7
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0. 05% BSA & 0. 05% azide.
Concentration
Purified Ab with BSA and Azide at 200ug/ml (varies by lot)
Applicable Applications for anti-COL4A1 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
alpha1 (IV)-alpha2(IV) triple helix. The epitope is triple helical domain about 900 from the N-terminus
Cellular Localization
Secreted > extracellular space > extracellular matrix > basement membrane
Positive Control
Human kidney, placenta or tonsil. Placenta tissue lysates.
Hu-chromosome Location
13q34 (COL4A1 & COL4A2)
Unigene
17441
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).)
IHC (Immunohistochemisry)
(Formalin-fixed, paraffin-embedded human kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embeddedhuman kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embeddedhuman kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human kidney adenocarcinoma. Strong staining of glomeruli using M3F7 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary negative control.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human kidney adenocarcinoma. Strong staining of glomeruli using M3F7 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary negative control.)
Related Product Information for anti-COL4A1 antibody
Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Collagen Type IV express at the basement membranes in a variety of tissues including kidney, muscle, lymph nodes, lung, tendon and spleen. Collagen Type IV has been shown to be useful in differentiating microinvasive from in situ ductal carcinomas of the breast. Other Collagen Type IV studies include use in pancreatic adenocarcinoma and chronic pancreatitis, nephrosclerosis and other kidney diseases, oral squamous cell carcinoma, laryngeal cancers, ovarian cancers and cervical cancers. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. M3F7 recognizes type IV collagen in basement membranes in kidney, lung, placenta, cornea and skin. This antibody does not recognize denatured type IV collagen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
160,615 Da
NCBI Official Full Name
collagen alpha-1(IV) chain preproprotein
NCBI Official Synonym Full Names
collagen, type IV, alpha 1
NCBI Official Symbol
COL4A1
NCBI Official Synonym Symbols
ICH; HANAC; POREN1; arresten
NCBI Protein Information
collagen alpha-1(IV) chain; collagen alpha-1(IV) chain; COL4A1 NC1 domain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain
UniProt Protein Name
Collagen alpha-1(IV) chain
UniProt Gene Name
COL4A1
UniProt Entry Name
CO4A1_HUMAN
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Product Notes
The COL4A1 col4a1 (Catalog #AAA215561) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Collagen IV (COL4A1/COL4A2) MouseMonoclonal Antibody [Clone M3F7] reacts with Human, Rat and Quail and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen IV (COL4A1/COL4A2) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the COL4A1 col4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen IV (COL4A1/COL4A2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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