IHC (Immunohistochemistry)
(Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (AAA40961) at 20 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.)
Mouse COVID 19 Spike P681R Antibody [7E3C5] (Delta Variant) Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant)
The immunogen is located within 650-700 amino acids of SARS-CoV-2 Delta variant Spike protein.
IHC (Immunohistochemistry)
(Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (AAA40961) at 20 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.)
IHC (Immunohistochemistry)
(Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (AAA40961) at 20 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.)
ELISA
(Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide, but not WT peptide and Alpha variant spike S1 peptide)
ELISA
(Figure 3 ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT PeptideCoating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide, but not WT peptide and Alpha variant spike S1 peptide)
ELISA
(Figure 2 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.)
ELISA
(Figure 2 ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 ProteinCoating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.)
Application Data
(Figure 1 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISACoating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
Application Data
(Figure 1 SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISACoating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, PM-9680, 1 μg/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.)
NCBI and Uniprot Product Information
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Product Notes
The COVID-19 (Catalog #AAA40961) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant) reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike P681R Antibody [7E3C5] (Delta Variant) Coronavirus can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike P681R Antibody [7E3C5] (Delta Variant) Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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