Mouse COX IV Monoclonal Antibody | anti-COXIV antibody

COX IV Monoclonal Antibody (6C8)

Cat. Num. AAA30797

• Gene Names: COX4I1; COX4; COXIV; COX4-1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: COX IV, Antibody; COX IV Monoclonal Antibody (6C8); Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4I1; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; anti-COXIV antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Clone Number: 6C8
• Specificity: The antibody detects endogenous COX IV protein.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-COXIV antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000-3000; IF: 1:200; IHC: 1:50-300
• Immunogen: Recombinant Protein of Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.COX IV Monoclonal Antibody(6C8) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

Application Data

(Zhang. Yanqiong. et al. "A discovery of clinically approved formula FBRP for repositioning to treat HCC by inhibiting PI3K/AKT/NF-?B activation." Molecular Therapy-Nucleic Acids19 (2020): 890-904.)

WB (Western Blot)

(Western blot analysis of lysates from 1) COS7.2) 3T3.3) Hela cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody (Assay Biotech:C40022) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot)

(Western blot analysis of Hela. diluted at 1) 1:2000 2) 1:5000)

Related Product Information for anti-COXIV antibody

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes

Product Categories/Family for anti-COXIV antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502981
• NCBI GeneID: 1327
• NCBI Accession #: NP_001852.1
• NCBI GenBank Nucleotide #: NM_001861.3
• UniProt Accession #: P13073; Q6P666; B2R4J2; D3DUM7
• Molecular Weight: 19,577 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit IV isoform 1
• NCBI Official Symbol: COX4I1
• NCBI Official Synonym Symbols: COX4; COXIV; COX4-1
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX IV-1; cytochrome c oxidase polypeptide IV
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Gene Name: COX4I1
• UniProt Synonym Gene Names: COX4; COX IV-1
• UniProt Entry Name: COX41_HUMAN