Mouse CPS1 / Carbamoyl-Phosphate Synthetase Monoclonal Antibody | anti-CPS1 antibody

CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62738

• Gene Names: CPS1; PHN; CPSASE1
• Reactivity: Human, Dog; Others not known.
• Applications: Immunohistochemistry, Immunofluorescence
• Synonyms: CPS1 / Carbamoyl-Phosphate Synthetase, Antibody; CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Mouse Monoclonal Antibody; Carbamoyl-phosphate synthetase 1 (CPS1); Carbamoylphosphate synthetase 1; CPSase 1; CPSASE1; anti-CPS1 antibody

• Host: Mouse
• Reactivity: Human, Dog; Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SPM615
• Specificity: This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCCs occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. ; Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. ; Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1506

• Applicable Applications for anti-CPS1 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence)
• Cellular Localization: Finely granular Cytoplasmic
• Immunogen: Recombinant human CPS1 protein
• Hu-Chromosome Location: 2q35
• Positive Control: HeLa cells. Liver or Hepatocellular Carcinoma (HCC)
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with CPS1 Monoclonal Antibody (SPM615).)

References

Haraguchi, Y., et al. 1991. Cloning and sequence of a cDNA encoding human carbamyl phosphate synthetase I: molecular analysis of hyperam- monemia. Gene 107: 335-340. Ramos-Vara, J.A., et al. Histochem 2002; J. 34: 397-401. Fan, Z., et al. Mod. Pathol 2003; 16: 137-144, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 169790915
• NCBI GeneID: 1373
• NCBI Accession #: NP_001116105.1
• NCBI GenBank Nucleotide #: NM_001122633.2
• UniProt Accession #: P31327; O43774; Q53TL5; Q59HF8; Q7Z5I5; B7Z818; J3KQL0
• Molecular Weight: ~165kDa
• NCBI Official Full Name: carbamoyl-phosphate synthase
• NCBI Official Synonym Full Names: carbamoyl-phosphate synthase 1
• NCBI Official Symbol: CPS1
• NCBI Official Synonym Symbols: PHN; CPSASE1
• NCBI Protein Information: carbamoyl-phosphate synthase [ammonia], mitochondrial
• UniProt Protein Name: Carbamoyl-phosphate synthase [ammonia], mitochondrial
• UniProt Gene Name: CPS1
• UniProt Synonym Gene Names: CPSase I
• UniProt Entry Name: CPSM_HUMAN