IHC (Immunohistochemisry)
(CSPG4/NG2 Antibody-Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human CSPG4/NG2 Monoclonal Antibody | anti-CSPG4 antibody
CSPG4/NG2 Mouse anti-Human Monoclonal (aa559-776) Antibody
Gene Names
CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Protein A + Protein G affinity chromatography
Synonyms
CSPG4/NG2, Antibody; CSPG4/NG2 Mouse anti-Human Monoclonal (aa559-776) Antibody; PathPlus CSPG4/NG2 Antibody (aa559-776); CSPG4; MCSP; MEL-CSPG; MSK16; NG2; HMW-MAA; MCSPG; anti-CSPG4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
The antibody is a mouse monoclonal antibody raised against MCSP.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
PBS, pH7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CSPG4 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Target
Human CSPG4/NG2
Immunogen
Recombinant MCSP (His559-Pro776) expressed in E Coli.
Conjugation
Unconjugated
Epitope
aa559-776
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
IHC (Immunohistochemisry)
(CSPG4/NG2 Antibody-Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(CSPG4/NG2 Antibody-Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(CSPG4/NG2 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(CSPG4/NG2 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CSPG4/NG2 Antibody-Western blot of recombinant CSPG4/NG2.)
WB (Western Blot)
(CSPG4/NG2 Antibody-Western blot of recombinant CSPG4/NG2.)
Related Product Information for anti-CSPG4 antibody
NG2 antibody is an unconjugated mouse monoclonal antibody to human NG2 (CSPG4)(aa559-776). Validated for IHC and WB.
CSPG4 (Chondroitin sulfate proteoglycan 4, MCSP, NG2) is a transmembrane proteoglycan that functions in angiogenesis, cell survival and migration, and the modulation of neuronal networks and pericyte functions. CSPG4 is an important prognostic marker in cancer, as it is upregulated in glioblastoma and is expressed on the cell surface of malignant melanoma cells, where it is involved in progression and spreading. In normal tissue, CSPG4 is highly expressed on NG2-glia cells in the brain, and is also found on osteoblasts, melanocytes, smooth muscle cells, mesenchymal stem cells, and pericytes. It is also a useful marker for oligodendrocyte precursor cells in developing and adult humans. Mutations in CSPG4 can result in a disruption in white matter integrity and oligodendrocyte morphology in the brain and are correlated with familial schizophrenia. In immunohistochemistry, CSGP4 has membranous and cytoplasmic positivity in all tissues throughout the body.
CSPG4 (Chondroitin sulfate proteoglycan 4, MCSP, NG2) is a transmembrane proteoglycan that functions in angiogenesis, cell survival and migration, and the modulation of neuronal networks and pericyte functions. CSPG4 is an important prognostic marker in cancer, as it is upregulated in glioblastoma and is expressed on the cell surface of malignant melanoma cells, where it is involved in progression and spreading. In normal tissue, CSPG4 is highly expressed on NG2-glia cells in the brain, and is also found on osteoblasts, melanocytes, smooth muscle cells, mesenchymal stem cells, and pericytes. It is also a useful marker for oligodendrocyte precursor cells in developing and adult humans. Mutations in CSPG4 can result in a disruption in white matter integrity and oligodendrocyte morphology in the brain and are correlated with familial schizophrenia. In immunohistochemistry, CSGP4 has membranous and cytoplasmic positivity in all tissues throughout the body.
References
Cellular & Molecular Biology Letters. 2017. 22(4), PMID: 28536635; Int J Mol Sci. 2018 Sep; 19(9): 2724, PMID: 30213051; Mol Psychiatry. 2019 May;24(5):757-771, PMID: 29302076
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
250537 MW
NCBI Official Full Name
chondroitin sulfate proteoglycan 4
NCBI Official Synonym Full Names
chondroitin sulfate proteoglycan 4
NCBI Official Symbol
CSPG4
NCBI Official Synonym Symbols
NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
NCBI Protein Information
chondroitin sulfate proteoglycan 4
UniProt Protein Name
Chondroitin sulfate proteoglycan 4
UniProt Gene Name
CSPG4
UniProt Synonym Gene Names
MCSP
UniProt Entry Name
CSPG4_HUMAN
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Product Notes
The CSPG4 cspg4 (Catalog #AAA162787) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CSPG4/NG2 Mouse anti-Human Monoclonal (aa559-776) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSPG4/NG2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CSPG4 cspg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSPG4/NG2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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