IHC (Immunohistochemisry)
Mouse Cyclin A Monoclonal Antibody | anti-CCNA2 antibody
Cyclin A
Gene Names
CCNA2; CCN1; CCNA
Reactivity
Human, Mouse, Cow, African Green Monkey, Mink (Mustela).
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
Cyclin A, Antibody; Cyclin A; CCN1; CCNA; CCNA2; CCNA2_HUMAN; Cyclin A2; anti-CCNA2 antibody
Host
Mouse
Reactivity
Human, Mouse, Cow, African Green Monkey, Mink (Mustela).
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
E67
Specificity
It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
432
Applicable Applications for anti-CCNA2 antibody
IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
Localization
Predominantly nuclear
Positive Control
K-562 and HeLa Cells. Human tonsil or lymph node
Human Chromosomal Location
4q27
Immunogen
Full length bovine Cyclin A protein
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemisry)
IHC (Immunohistochemisry)
WB (Western Blot)
WB (Western Blot)
SDS-PAGE
(SDS-PAGE Analysis Purified Cyclin A2 Mouse Monoclonal Antibody (E67). Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Cyclin A2 Mouse Monoclonal Antibody (E67). Confirmation of Integrity and Purity of Antibody.)
References
1. Adamczewski JP; Gannon JV; Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 1993, 67(11):6551-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54kDa
NCBI Official Full Name
cyclin-A2
NCBI Official Synonym Full Names
cyclin A2
NCBI Official Symbol
CCNA2
NCBI Official Synonym Symbols
CCN1; CCNA
NCBI Protein Information
cyclin-A2
UniProt Protein Name
Cyclin-A2
UniProt Gene Name
CCNA2
UniProt Synonym Gene Names
CCN1; CCNA; Cyclin-A
UniProt Entry Name
CCNA2_HUMAN
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Product Notes
The CCNA2 ccna2 (Catalog #AAA62599) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cyclin A reacts with Human, Mouse, Cow, African Green Monkey, Mink (Mustela). and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CCNA2 ccna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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