WB (Western Blot)
(Detection of human CCNA2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCNA2 antibody AAA213175 (lot AAA213175-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human CCNA2/Cyclin A2 Polyclonal Antibody | anti-CCNA2/CyclinA2 antibody
Rabbit anti-CCNA2/Cyclin A2 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CCNA2/Cyclin A2, Antibody; Rabbit anti-CCNA2/Cyclin A2 Antibody, Affinity Purified; CCNA; Cyclin A; cyclin-A; cyclin-A2; CCN1; Cyclin-A2; cyclin A2; anti-CCNA2/CyclinA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CCNA2/CyclinA2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 100 and 150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CCNA2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCNA2 antibody AAA213175 (lot AAA213175-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human CCNA2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCNA2 antibody AAA213175 (lot AAA213175-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CCNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNA2 antibody AAA213175 (lot AAA213175-1) used for IP at 6 ug per reaction. CCNA2 was also immunoprecipitated by rabbit anti-CCNA2 antibody For blotting immunoprecipitated CCNA2, AAA213175 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CCNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNA2 antibody AAA213175 (lot AAA213175-1) used for IP at 6 ug per reaction. CCNA2 was also immunoprecipitated by rabbit anti-CCNA2 antibody For blotting immunoprecipitated CCNA2, AAA213175 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-CCNA2/CyclinA2 antibody
The protein encoded by the gene CCNA2, Cyclin A2, belongs to the highly conserved cyclin family. Members of the cyclin family are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, Cyclin A2 is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions [taken from NCBI Entrez Gene ID: 890].
Product Categories/Family for anti-CCNA2/CyclinA2 antibody
NCBI and Uniprot Product Information
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Product Notes
The CCNA2/CyclinA2 ccna2 (Catalog #AAA213175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CCNA2/Cyclin A2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCNA2/Cyclin A2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCNA2/CyclinA2 ccna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNA2/Cyclin A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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