Mouse anti-Human Cyclin A1 Monoclonal Antibody | anti-CCNA1 antibody

Anti-Cyclin A1 Antibody, Mouse Monoclonal

Cat. Num. AAA255289

• Gene Names: CCNA1; CT146
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: Cyclin A1, Antibody; Anti-Cyclin A1 Antibody, Mouse Monoclonal; Cyclin A1/CCNA1 Antibody, Mouse MAb; cyclin A1; Anti-CT146 Antibody; anti-CCNA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 06
• Specificity: Human Cyclin A1/CCNA1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CCNA1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant Human Cyclin A1/CCNA1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cyclin A1/CCNA1 (rh Cyclin A1/CCNA1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human CCNA1 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CCNA1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human CCNA1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCNA1 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.)

Related Product Information for anti-CCNA1 antibody

Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.

Product Categories/Family for anti-CCNA1 antibody

AMPK Signaling Pathway

References

Yang R, et al. (1999) Functions of Cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins. Molecular and Cellular biology. 19 (3): 2400-7.Yang R, et al. (1999) Cyclin A1 expression in leukemia and normal hematopoietic cells. Blood. 93 (6): 2067-74.

NCBI and Uniprot Product Information

• NCBI GI #: 4502611
• NCBI GeneID: 8900
• NCBI Accession #: NP_003905.1
• NCBI GenBank Nucleotide #: NM_003914.3
• UniProt Accession #: P78396; Q5T3V0; Q5U0G2; Q8IY91; B7Z7E3
• Molecular Weight: 47,494 Da
• NCBI Official Full Name: cyclin-A1 isoform a
• NCBI Official Synonym Full Names: cyclin A1
• NCBI Official Symbol: CCNA1
• NCBI Official Synonym Symbols: CT146
• NCBI Protein Information: cyclin-A1
• UniProt Protein Name: Cyclin-A1
• UniProt Gene Name: CCNA1
• UniProt Entry Name: CCNA1_HUMAN