WB (Western Blot)
(Anti-CCNA1 rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:65 kDaObserved band size:85 kDa)
Rabbit anti-Human Cyclin A1 Polyclonal Antibody | anti-CCNA1 antibody
Anti-Cyclin A1 Antibody, Rabbit Polyclonal
Gene Names
CCNA1; CT146
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Cyclin A1, Antibody; Anti-Cyclin A1 Antibody, Rabbit Polyclonal; Cyclin A1/CCNA1 Antibody, Rabbit PAb, Antigen Affinity Purified; cyclin A1; Anti-CT146 Antibody; anti-CCNA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Cyclin A1/CCNA1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CCNA1 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Cyclin A1/CCNA1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Cyclin A1/CCNA1 (rh Cyclin A1/CCNA1). Cyclin A1/CCNA1 specific IgG was purified by Human Cyclin A1/CCNA1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-CCNA1 rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:65 kDaObserved band size:85 kDa)
WB (Western Blot)
(Anti-CCNA1 rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:65 kDaObserved band size:85 kDa)
IP (Immunoprecipitation)
(CCNA1 was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate0.5 uL anti-CCNA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCNA1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
IP (Immunoprecipitation)
(CCNA1 was immunoprecipitated using:Lane A:0.5 mg THP1 Whole Cell Lysate0.5 uL anti-CCNA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCNA1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
Related Product Information for anti-CCNA1 antibody
Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.
Product Categories/Family for anti-CCNA1 antibody
References
Yang R, et al. (1999) Functions of Cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins. Molecular and Cellular biology. 19 (3): 2400-7.Yang R, et al. (1999) Cyclin A1 expression in leukemia and normal hematopoietic cells. Blood. 93 (6): 2067-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,494 Da
NCBI Official Full Name
cyclin-A1 isoform a
NCBI Official Synonym Full Names
cyclin A1
NCBI Official Symbol
CCNA1
NCBI Official Synonym Symbols
CT146
NCBI Protein Information
cyclin-A1
UniProt Protein Name
Cyclin-A1
UniProt Gene Name
CCNA1
UniProt Entry Name
CCNA1_HUMAN
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Product Notes
The CCNA1 ccna1 (Catalog #AAA255290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin A1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin A1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CCNA1 ccna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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