Mouse CYCS Monoclonal Antibody | anti-CYCS antibody

CYCS Monoclonal Antibody (4B10)

Cat. Num. AAA30821

• Gene Names: CYCS; CYC; HCS; THC4
• Reactivity: Human, Mouse, Rat, Chicken
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CYCS, Antibody; CYCS Monoclonal Antibody (4B10); Cytochrome c; CYCS; CYC; anti-CYCS antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Chicken
• Clonality: Monoclonal
• Clone Number: 4B10
• Specificity: The antibody detects endogenous CYCS protein.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-CYCS antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-5000; IHC: 1:500-1000; IF: 1:200
• Immunogen: Recombinant Protein of CYCS
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Rat-liver tissue. 1.CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-spleen tissue. 1.CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-liver-cancer tissue. 1.CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.c-Myc Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). CYCS Monoclonal Antibody(4B10)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CYCS Monoclonal Antibody(4B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS Monoclonal Antibody.)

WB (Western Blot)

(Western blot analysis of lysates from 1) Hela. 2) 293T. 3) 3T3. cells. ?Green? primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. ?Red? GAPDH Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot)

(Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000)

WB (Western Blot)

(Western blot analysis of 1) Hela. 2) 293T. 3) 3T3. 4) Mouse Liver Tissue. 5) Rat Liver Tissue. 6) Rat Kidney Tissue using CYCS Monoclonal Antibody.)

Related Product Information for anti-CYCS antibody

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Product Categories/Family for anti-CYCS antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 11128019
• NCBI GeneID: 54205
• NCBI Accession #: NP_061820.1
• NCBI GenBank Nucleotide #: NM_018947.5
• UniProt Accession #: P99999; P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1
• Molecular Weight: 11,749 Da
• NCBI Official Full Name: cytochrome c
• NCBI Official Synonym Full Names: cytochrome c, somatic
• NCBI Official Symbol: CYCS
• NCBI Official Synonym Symbols: CYC; HCS; THC4
• NCBI Protein Information: cytochrome c
• UniProt Protein Name: Cytochrome c
• UniProt Gene Name: CYCS
• UniProt Synonym Gene Names: CYC
• UniProt Entry Name: CYC_HUMAN