IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil, using Cytokeratin 13 Antibody.)
Rabbit anti-Human, Mouse Cytokeratin 13 Monoclonal Antibody | anti-KRT13 antibody
Anti-Cytokeratin 13 Rabbit Monoclonal Antibody
Gene Names
KRT13; K13; CK13; WSN2
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Affinity-chromatography
Synonyms
Cytokeratin 13, Antibody; Anti-Cytokeratin 13 Rabbit Monoclonal Antibody; Keratin, type I cytoskeletal 13; Cytokeratin-13; CK-13; Keratin-13; K13; KRT13; anti-KRT13 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
GAC-11
Purity/Purification
Affinity-chromatography
Form/Format
Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Sequence Length
420
Applicable Applications for anti-KRT13 antibody
WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human Cytokeratin 13
Preparation and Storage
Store at -20°C.Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil, using Cytokeratin 13 Antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil, using Cytokeratin 13 Antibody.)
WB (Western Blot)
(Western blot analysis of Cytokeratin 13 expression in A431 cell lysate (AAA124471).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT13 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT13)
WB (Western Blot)
(Western blot analysis of Cytokeratin 13 expression in A431 cell lysate (AAA124471).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT13 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT13)
Related Product Information for anti-KRT13 antibody
Anti-Cytokeratin 13 Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human, Mouse
Product Categories/Family for anti-KRT13 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 13 isoform b
NCBI Official Synonym Full Names
keratin 13
NCBI Official Symbol
KRT13
NCBI Official Synonym Symbols
K13; CK13; WSN2
NCBI Protein Information
keratin, type I cytoskeletal 13
UniProt Protein Name
Keratin, type I cytoskeletal 13
UniProt Gene Name
KRT13
UniProt Synonym Gene Names
CK-13; K13
Similar Products
Product Notes
The KRT13 krt13 (Catalog #AAA124471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 13 Rabbit Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 13 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the KRT13 krt13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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