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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse Cytokeratin 20 (KRT20) Monoclonal Antibody | anti-KRT20 antibody
Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human. Others not known.
Applications
Immunohistochemistry, Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Cytokeratin 20 (KRT20), Antibody; Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker); CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20; anti-KRT20 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
KRT20/1993
Specificity
This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
424
Applicable Applications for anti-KRT20 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
Positive Control
HT29 Cells. Colon Carcinoma
Cellular Localization
Cytoplasmic
Chromosome Location
17q21.2
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
WB (Western Blot)
(Western Blot Analysis of human HT29 Cell Lysate using Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1993).)
WB (Western Blot)
(Western Blot Analysis of human HT29 Cell Lysate using Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1993).)
SDS-PAGE
(SDS-PAGE Analysis Purified Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1993). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1993). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Normal Colon stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Normal Colon stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1993).)
References
Moll, R., et al. 1993. The human gene encoding Cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation 53: 75-93.; Wauters, C.C., et al. 1995. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum. Pathol. 26: 852-855.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46kDa
NCBI Official Full Name
keratin, type I cytoskeletal 20
NCBI Official Synonym Full Names
keratin 20
NCBI Official Symbol
KRT20
NCBI Official Synonym Symbols
K20; CD20; CK20; CK-20; KRT21
NCBI Protein Information
keratin, type I cytoskeletal 20
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
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Product Notes
The KRT20 krt20 (Catalog #AAA214795) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 20 (KRT20) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT20 krt20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 20 (KRT20), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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