FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-Cytokeratin 7 antibody OV-TL 12/30 . HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of OV-TL 12/30 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Mouse anti-Human Cytokeratin 7 Monoclonal Antibody | anti-KRT7 antibody
Anti-Cytokeratin 7 [OV-TL 12/30]
Gene Names
KRT7; K7; CK7; SCL; K2C7
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot
Purity
Purified antibody. Protein A affinity purified
Synonyms
Cytokeratin 7, Antibody; Anti-Cytokeratin 7 [OV-TL 12/30]; CK-7; Keratin, type II cytoskeletal 7; Cytokeratin-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7; CK7; CK 7; anti-KRT7 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
OV-TL 12/30
Specificity
This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not Recognise other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-KRT7 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot)
PMID
1690512
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-Cytokeratin 7 antibody OV-TL 12/30 . HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of OV-TL 12/30 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-Cytokeratin 7 antibody OV-TL 12/30 . HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of OV-TL 12/30 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
WB (Western Blot)
(Western Blot using anti- Cytokeratin 7 antibody OV-TL 12/30 HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti- Cytokeratin 7 antibody OV-TL 12/30 HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed HeLa cells with anti-Cytokeratin 7 antibody OV-TL 12/30 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of OV-TL 12/30 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed HeLa cells with anti-Cytokeratin 7 antibody OV-TL 12/30 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of OV-TL 12/30 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-KRT7 antibody
Recombinant monoclonal antibody to Cytokeratin 7. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OV-TL 12/30.
This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.
This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.
Product Categories/Family for anti-KRT7 antibody
References
Ramaekers et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar; 136(3): 641-655.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,386 Da
NCBI Official Full Name
keratin, type II cytoskeletal 7
NCBI Official Synonym Full Names
keratin 7
NCBI Official Symbol
KRT7
NCBI Official Synonym Symbols
K7; CK7; SCL; K2C7
NCBI Protein Information
keratin, type II cytoskeletal 7; CK-7; keratin-7; sarcolectin; cytokeratin 7; cytokeratin-7; type-II keratin Kb7; type II mesothelial keratin K7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7
UniProt Protein Name
Keratin, type II cytoskeletal 7
UniProt Gene Name
KRT7
UniProt Synonym Gene Names
SCL; CK-7; K7
UniProt Entry Name
K2C7_HUMAN
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Product Notes
The KRT7 krt7 (Catalog #AAA72181) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 7 [OV-TL 12/30] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT7 krt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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