Mouse Cytokeratin 8 (KRT8) Monoclonal Antibody | anti-KRT8 antibody

Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody

Cat. Num. AAA62584

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human, Rat, Zebrafish
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Cytokeratin 8 (KRT8), Antibody; Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody; CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; KRT8; Type-II Keratin Kb8; anti-KRT8 antibody

• Host: Mouse
• Reactivity: Human, Rat, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: H1
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 483

• Applicable Applications for anti-KRT8 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic
• Immunogen: Cytoskeleton preparation containing cytokeratin 8
• Hu-Chromosome Location: 12q13.13
• Positive Control: MCF-7, HeLa, A431 cells. Skin, Colon, lung or breast carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence Analysis of MCF-7 Cells labeling CK8 with Cytokeratin 8 Mouse Monoclonal Antibody (H1) followed by Goat anti-mouse IgG-CF488(Green). The nuclear counterstain is Reddot. (Red))

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of HeLa Cells.Using Cytokeratin 8 Mouse Monoclonal Antibody (H1) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red))

WB (Western Blot)

(Western Blot Analysis of A431 Cell Lysate using Cytokeratin 8 Monoclonal Antibody (H1).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (H1).)

References

Guelstein VI et. al. Int J Cancer 42:147-53 (1988)

NCBI and Uniprot Product Information

• NCBI GI #: 372466577
• NCBI GeneID: 3856
• NCBI Accession #: NP_001243222.1
• NCBI GenBank Nucleotide #: NM_001256293.1
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
• Molecular Weight: 52.5kDa
• NCBI Official Full Name: keratin, type II cytoskeletal 8 isoform 2
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN