WB (Western Blot)
(The cell lysate derived from 1) SW620, 2) Hela cells were immunoprobed by Mouse anti-CK8(AAA71350) at 1:500. An immunoreactive band is observed around ~58kDa.)
Mouse Keratin 8 (pS431) Monoclonal Antibody | anti-KRT8 antibody
Mouse Anti Keratin 8 (pS431) Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein A Chromatography
Synonyms
Keratin 8 (pS431), Antibody; Mouse Anti Keratin 8 (pS431) Monoclonal Antibody; CK-8; cytokeratin 8; anti-KRT8 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Protein A Chromatography
Form/Format
This affinity purified antibody is supplied in Sterile Phosphate Buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-KRT8 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Antigen Preparation
A synthetic peptide derived phosphorylated serine 431 of Human cytokeratin 8.
Specificity
This antibody recognizes phosphorylated pS431 of Keratin 8. The other species are not tested.
Cellular Location
Cytoplasm
Positive Control
SW620
Preparation and Storage
The antibodies are stable for up to 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2 to 8°C for up to three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
WB (Western Blot)
(The cell lysate derived from 1) SW620, 2) Hela cells were immunoprobed by Mouse anti-CK8(AAA71350) at 1:500. An immunoreactive band is observed around ~58kDa.)
WB (Western Blot)
(The cell lysate derived from 1) SW620, 2) Hela cells were immunoprobed by Mouse anti-CK8(AAA71350) at 1:500. An immunoreactive band is observed around ~58kDa.)
Related Product Information for anti-KRT8 antibody
Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 8 and 18 (K8/18) phosphorylation, such as K8 (S23, S73, S431) and K18 (S33, S52), plays a significant and site-specific role in regulating keratin filament organization, association with binding proteins, and modulation of cell cycle progression. Keratin hyperphosphorylation correlates with exposure to a variety of stresses in cultured cells and in mouse models of liver, pancreatic, and gallbladder injury, and it is found in assocation with mouse and human Mallory bodies. The increase of site-specific keratin phosphorylation in liver disease might be a progression marker.
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Product Notes
The KRT8 (Catalog #AAA71350) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Keratin 8 (pS431) Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 8 (pS431) can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 8 (pS431), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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